A case of ovarian juvenile granulosa cells tumor with peritoneal and jejunal localisation at Amath Dansokho Regional Hospital, Kedougou, Senegal

Granulosa tumors are rare ovarian tumors they belong to sex-cord and stromal tumor family. They are the most common type of malignant stromal tumor and have a good prognosis. There are two types: the adult type (AGCTs), which occurs most frequently between the ages of 40 and 70, and the juvenile type (JGCTs), which is uncommon. Juvenile granulosa tumors tend to involve a single ovary and occur mainly in people who are younger than 30 years. Metastatic spread is rare and, if present, is usually limited to the peritoneal cavity. We report a case of ovarian juvenile granulosa cell tumor extending to the jejunum and peritoneum in an 18-year-old female patient received in our institution.

Replication of SARS-CoV-2 in cell lines used in public health surveillance programmes with special emphasis on biosafety

Background & objectives: Polio, measles, rubella, influenza and rotavirus surveillance programmes are of great public health importance globally. Virus isolation using cell culture is an integral part of such programmes. Possibility of unintended isolation of SARS-CoV-2 from clinical specimens processed in biosafety level-2 (BSL-2) laboratories during the above-mentioned surveillance programmes, cannot be ruled out. The present study was conducted to assess the susceptibility of different cell lines to SARS- CoV-2 used in these programmes. Methods: Replication of SARS-CoV-2 was studied in RD and L20B, Vero/hSLAM, MA-104 and Madin–Darby Canine Kidney (MDCK) cell lines, used for the isolation of polio, measles, rubella, rotavirus and influenza viruses, respectively. SARS-CoV-2 at 0.01 multiplicity of infection was inoculated and the viral growth was assessed by observation of cytopathic effects followed by real-time reverse transcription–polymerase chain reaction (qRT-PCR). Vero CCL-81 cell line was used as a positive control. Results: SARS-CoV-2 replicated in Vero/hSLAM, and MA-104 cells, whereas it did not replicate in L20B, RD and MDCK cells. Vero/hSLAM, and Vero CCL-81 showed rounding, degeneration and detachment of cells; MA-104 cells also showed syncytia formation. In qRT-PCR, Vero/hSLAM and MA-104 showed 106 and Vero CCL-81 showed 107 viral RNA copies per ?l. The 50 per cent tissue culture infectious dose titres of Vero/hSLAM, MA-104 and Vero CCL-81 were 105.54, 105.29 and 106.45/ml, respectively. Interpretation & conclusions: Replication of SARS-CoV-2 in Vero/hSLAM and MA-104 underscores the possibility of its unintended isolation during surveillance procedures aiming to isolate measles, rubella and rotavirus. This could result in accidental exposure to high titres of SARS-CoV-2, which can result in laboratory acquired infections and community risk, highlighting the need for revisiting biosafety measures in public health laboratories