Study of mycelial polysaccharide from Paraisaria dubia of Ophiocordyceps gracilis asexual / 中国中药杂志

In order to provide a foundation for the development and application of Ophiocordyceps gracilis and increase the new resources of cordyceps,an asexual Paraisaria dubia was isolated from an O. gracilis fruit body. After 10 days of liquid fermentation,white globular mycelium and clear transparent fermentation were produced. The mycelium was extracted by hot water and precipitated with ethanol to obtain intracellular crude polysaccharide. The protein was deproteinized to obtain deproteinized polysaccharide. The intracellular pure polysaccharide was purified by Sepharose 4 B column chromatography and were analyzed by UV,IR,1 H-NMR,and13 CNMR data,as well as GC and HPLC. The results showed that the intracellular polysaccharide of P. dubia was composed of glucose,galactose and mannose with a molar ratio of 25. 54 ∶2 ∶1. It was a β-configuration glycosylic bond,containing pyranoside. The initial connection of polysaccharide was β(1→2)(1→4)(1→6) connection. This experiment provides a theoretical basis for the development and application of P. dubia.

Optimum Culture Conditions for Bacterial Cellulose Produced by Acetobacter xylinum NUST4 / 微生物学通报

The optimum culture conditions for Acetobacter xylinum NUST4 which produces bacterial cellulose(BC) were obtained by uniform design method.BC production was dependent on MgSO_(4)?7H_(2)O,FeSO_(4)?7H_(2)O and cosubstrates such as p-aminobenzoic acid,nicotinamide,d-Biotin and ethanol.The optimal medium contained glucose 24g,sucrose 22g,peptone 16g,HAc 2.4mL,Na_(2)HPO_(4)?12H_(2)O 3.5 g,KH_(2)PO_(4)?H_(2)O 1 g,MgSO_(4)?7H_(2)O 6 g,FeSO_(4)?7H_(2)O 0.015g,nicotinamide 0.003 g,d-Biotin 0.02g and ethanol 20mL in 1L culture medium.BC yield reached 9.87g(the dried weight) in stationary culture for 7 days,which was 12-fold higher than that in the S-H medium.

Study on the Sterilization Effects on Ganoderma lucidum Hyphae by the Treatment of Ozone / 微生物学通报

The mortality rate was measured against the time of the treatment of ozone and the variation of ozone concentration. Experiment results indicate that Ganoderma lucidum hyphae could be destroyed completely by ozone and the mortality rate obviously went up with the prolonging of treatment time. The changes of cell form and the fluorescence of hyphae (dyed by acridine orange or not) were observed through biological microscope, laser confocal scanning microscopic imaging system, transmission electron microscope, scanning electron microscope and atom force microscope, and the mechanism of Ganoderma lucidum hyphae (eucaryote) destroyed by ozone was studied.