RESUMO
To investigate the adjuvant effect of granulocyte macrophage colony stimulating factor (GM-CSF) in Flaviviridae virus DNA vaccines. After DNA immunization, the antibody levels of serum from mice were detected by ELISA and indirect immunofluorescence assay. Co-immunization of GM-CSF suppressed the immune responses induced by DV1 and DV2 candidate vaccines whereas enhanced the immune response induced by HCV C and E1 DNA vaccines. As genetic adjuvant for DNA vaccines, GM-CSF might display complex diversity on the immune responses: an augmentation or suppression due to different immunogens. Therefore, GM-CSF should be used with some cautions in clinic.
Assuntos
Animais , Feminino , Humanos , Camundongos , Adjuvantes Imunológicos , Anticorpos Antivirais , Alergia e Imunologia , DNA Viral , Genética , Alergia e Imunologia , Dengue , Alergia e Imunologia , Virologia , Vacinas contra Dengue , Genética , Alergia e Imunologia , Vírus da Dengue , Genética , Alergia e Imunologia , Fator Estimulador de Colônias de Granulócitos e Macrófagos , Alergia e Imunologia , Hepacivirus , Genética , Alergia e Imunologia , Hepatite C , Alergia e Imunologia , Virologia , Imunização , Camundongos Endogâmicos BALB C , Vacinas de DNA , Genética , Alergia e Imunologia , Vacinas Virais , Genética , Alergia e ImunologiaRESUMO
We investigated the causative agents of hand, foot and mouth disease (HFMD) in children in Beijing epidemic from April to June of 2007. Totally, 82 specimens (including throat swabs, rectal swabs and vesicular swabs) were collected from 51 patients with HFMD. All patients had typical skin lesions, but no neurological manifestations. These clinical specimens were directly tested by RT-PCR assay with three primer sets: universal enterovirus, CA16-specific and EV71-specific primers. Enterovirus, CA16 and EV71 were identified depending on the size of PCR products. The percentage of the specimens identified as positive for enterovirus was 70.6%. Of the 51 cases, 25 were positive for CA16, 4 were positive for EV71, and 7 were enterovirus positive of non-CA16 or EV71. The ratio among them was about 6:1:2. EV71 strains belonged to genotype C4 after sequencing and phylogenetic analysis.
Assuntos
Pré-Escolar , Feminino , Humanos , Masculino , China , Enterovirus , Classificação , Genética , Fisiologia , Genótipo , Doença de Mão, Pé e Boca , Virologia , Filogenia , Reação em Cadeia da Polimerase Via Transcriptase ReversaRESUMO
Thermal Shift Assays was applied for high-throughput screening optimized buffer system used in the gel filtration chromatography for purification of recombinant TBEV serine protease. The optimization included kinds of salt and pH. It was optimized new buffer remarkably improved the quality of the purified TBEV serine protease by increasing its monomer from 75% to 99%. This result may improve future crystal studies of this protein.