Effect of ginsenoside Rh2 on immunocompetence of alveolar macrophages in patients with non-small cell lung cancer / 中南大学学报(医学版)

OBJECTIVE@#To explore the effect of ginsenoside Rh2 (G-Rh2) on the excretion of cytotoxin-effecting molecule of alveolar macrophages (AM) in patients with non-small cell lung cancer (NSCLC).@*METHODS@#The concentration of tumor necrosis factor (TNF-alpha) and NO in the bronchoalveolar lavage fluid (BALF) and the cultured supernatants of AM in 35 patients with NSCLC were measured by ELISA and enzyme method,and levels of TNF-alpha and NO in the cultured supernatants of AM after being cultivated with IFN-alpha, G-Rh2, and IFN-alpha+G-Rh2 were measured by the same method.@*RESULTS@#AM in all the non-small cell lung cancer patients produced TNF-alpha and NO. The activity of TNF-alpha and NO was lower in the BALF and in the cultured supernatants of AM of the tumor-bearing lungs than that of the non-tumor-bearing lungs. The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with G-Rh2 were higher than those in the control (P0.05). The concentrations of TNF-alpha and NO in the cultured supernatants of AM cultivated with both G-Rh2 and IFNalpha were obviously higher than those stimulated with IFNalpha or G-Rh2 (P<0.01) alone.@*CONCLUSION@#G-Rh2 can enhance the excretion of cytotoxin-effecting molecules of AM in patients with NSCLC. The changes are more distinctive when G-Rh2 and IFNalpha have coordinated action.

Study on the feasibility of using magnetic iron oxide nanoparticles as wt-p 53 gene carrier for transfection of lung cancer cells / 肿瘤

Objective: To evaluate the feasibility of using magnetic iron oxide nanoparticles (pll-DCIONP) with poly-L-lysine modified surface as wild type p 53 gene carrier for transfection of lung cancer cells in vitro. Methods: The dextran-coated iron oxide nanoparticles (DCIONP) were synthesized with chemical precipitation method. The surface of DCIONP was modified by self-assembled poly-L-lysine to form particle complexes (pll-DCIONP). The configuration of pll-DCIONP was detected by scanning electron microscope. The diameter, size distribution and Zeta surface potential of pll-DCIONP was detected by Laser Particle Size Analyzer. The potential of adsorbing wt-p 53 gene and resisting DNase- I and blood serum digestion of pll-DCIONP/wt-p53 complex were analyzed by spectrophotometer and agarose gel electrophoresis, respectively. The pll-DCIONP was evaluated as a kind of wt-p53 gene carrier and transfected into human lung adenocarcinoma cell line A549/DDP in vitro. The mRNA and protein expression of intracellular p53 were tested by RT-PCR and Western blotting, respectively. Results: The diameter of the pll-DCIONP was between 60 and 80 nm. The Zeta surface potential of pll-DCIONP was + 19. 6 mV. At different pH conditions, the pll-DCIONP could adsorb wt-p 53 gene which was the strongest when the ratio of pII-DCIONP/wt-p53 was 1: 1 (m/m). The pll-DCIONP/wt-p 53 complex could resist DNase-I and blood serum digestion. The intracellular p 53 gene levels continuously increased in a time-dependent manner after transfection of human lung adenocarcinoma cells with pll-DCIONP/wt-p53 complex. The intracellular p 53 gene levels decreased in a time-dependent manner after transfection with the wt-p 53 DNA-lipofectamine complex. Conclusion: pll-DCIONP could be used as one of the ideal gene carriers for wt-p 53 gene delivery. It continuously and effectively mediates wt-p 53 gene transfection into lung cancer cells.