RESUMO
AIM: To investigate the effect of bone marrow (BM)-derived mesenchymal stem cells (MSCs) on the proliferation of hepatic stellate cells (HSCs) in vitro. METHODS: MSCs and HSCs were isolated from BM and liver respectively in Fischer 344 rats. HSCs were re-cultured on plastic and appeared activated morphologically. The coculture system was set up by transwell insert with pore size 0.4 ?m and 6-well plates. The 5th passage HSCs were cultured on the 6-well plastic plate and MSCs or buffalo rat liver cells (BRLs) or HSCs seeded on the transwell insert. Smooth muscle ?-actin (?-SMA) and desmin expression of HSCs were tested by immunocytochemistry and quantified by IBAS 2.5 software. RESULTS: After coculture for 24 h, the proliferation of HSCs was inhibited to a moderate extent by MSCs. With the coculture time lasted, the significant growth suppression of HSCs was affected by MSCs (15.7% and 30.3%) but not BRL cells in 48 h and in 72 h. After coculture for 72 h, the expression of ?-SMA in HSCs was lower in MSCs coculture system than that with BRLs or HSCs alone system (50.2% vs 90.2%, 95.6%, P
RESUMO
AIM: To investigate effects of rBMMSC on hematopoiesis and immune reconstitution after allo-hematopoietic stem cells transplantation (HSCT). METHODS: Allogeneic BMT model from Fischer344 rats (RT-1Al) to Wistar rats (RT-1Au) was established. The effects of MSCs on hematopoietic reconstitution and immune reconstitution were studied by observing the survival rate, peripheral blood counts, thymus counts, spleen counts, bone marrow counts and immune function analysis at 30 days after transplantation. RESULTS: 1. Cotransplantation of MSCs and bone marrow (BM) was demonstrated to improve hematopoietic reconstitution. Lymphocyte and platelet counts in peripheral blood in cotransplantation groups were higher than those in control groups. More bone marrow neucleated cells were also observed in cotransplantation groups. 2. Cotransplantation of MSCs and BM improved immune reconstitution. First, overall thymic cellularity and spleen cellularity significantly increased in cotransplantation groups at day 30. Secondly, cotransplantation improved immune functional recovery. Non-specific lymphocytes proliferation reaction induced by ConA and LPS increased in cotransplantation group, and so did for allogeneic mixed-lymphocyte reaction. CONCLUSION: Hematopoietic reconstitution and immune reconstitution were significantly enhanced by MSCs cotransplanted with BM.