Three-dimentional cultured adipose mesenchymal stem cells for alveolar cleft bone repair: an in vitro study / 中华医学美学美容杂志

Objective:To explore the osteogenic effect of adipose-derived mesenchymal stem cells (ADSCs) in three-dimensional culture conditions in Matrigel hydrogel to provide theoretical basis for tissue engineering bone repair of alveolar process fractures.Methods:This study was performed in the Laboratory of Guangzhou Women and Children Medical Center in June 2019. The fourth generation of ADSCs were used to adjust the cell density to 1×10 5/mL. Two-dimensional common culture media (group A) and two-dimensional osteogenic induction media (group B) were used. The ADSCs were encapsulated in hydrogel with osteogenic induction media (group C), and the proliferation of cells was detected by CCK8 method. The cell mineralization effect was detected by alizarin red staining and alkaline phosphatase activity. The expression levels of osteocalcin (OCN) and osteopontin (OPN) were detected by qRT-PCR and Western blot assay. Results:ADSCs were encapsulated in hydrogel. The results of CCK8 kit showed that ADSCs could stably proliferate under the three-dimensional culture. The alizarin red staining and alkaline phosphatase activity of ADSCs under three-dimensional culture conditions were significantly higher than those of two-dimensional condition. In the detection of Western blotting and RT-PCR, we found that the expression of osteogenic protein and mRNA (OCN and OPN) of ADSCs in three-dimensional culture was also higher than that of two-dimensional culture.Conclusions:The encapsulation of ADSCs in hydrogel does not affect the proliferative potential of stem cells, and 3D cultured stem cells can significantly enhance their osteogenesis in vitro.

Clinical practice of prophylactic nipple- sparing mastectomy and immediate reconstruction in Chinese healthy women with BRCA1/BRCA2 germline mutation / 中国肿瘤临床

Objective: To explore the possibility of prophylactic nipple-sparing mastectomy and immediate reconstruction in healthy Chinese women with BRCA1/BRCA2 germline mutation. Methods: From January 2018 to February 2019, three healthy Chinese women with a BRCA1/ BRCA2 germline pathogenic mutation were treated at the Department of Breast Surgery, Peking University International Hospital (2 cases of BRCA1 mutation and 1 case of BRCA2 mutation); these three women (aged 34-36 years) had a strong family history of breast cancer. Thus, they underwent prophylactic nipple-sparing mastectomy and immediate reconstruction. Results: The operations were successfully completed, and no complications occurred. After a median 18-month follow-up, no breast cancer occurred in the three women; they were satisfied with the reconstructed breast's shape, and their anxiety also significantly reduced. Conclusions: Healthy Chinese women with a BRCA1/ BRCA2 germline mutation and a strong family history of breast cancer can possibly consider prophylactic nipple-sparing mastectomy and immediate reconstruction as options.

Diagnostic value of ultrasound-guided 14 gauge-core needle biopsy in breast nodules / 中华普通外科杂志

Objective@#To analyze the diagnostic value of ultrasound guided 14 gauge coreneedle biopsy (US-CNB) in breast nodules.@*Methods@#We retrospectively analyzed the pathological results of US-CNB and surgical excision from 373 breast nodules in Peking University International Hospital from Sep 2016 to Nov 2018 to evaluate the accuracy of 14g US-CNB.@*Results@#A total of 349 patients(373 nodules)underwent US-CNB. US-CNB reported 282 benign lesions(75.6%, 282/373), 20 high-risklesions(5.4%, 20/373), and 71 malignant lesions(19.0%, 71/373). For 282 CNB reported benign lesions, the surgical pathology confirmed 235 lesions , 46 for high-risk lesions and 1 for malignant lesion with a concordancy of 83.3%(235/282)and the underestimation rate was 16.7%(47/282). US-CNB identified 20 high-risk lesions. According to surgical results, 15 were high-risk lesions and 5 were malignant lesions with a concordancy of 75% (15/20)and the underestimation rate was 25%(5/20). When it comes to malignant lesions, the excision results showed that 70 were malignant lesions and 1was high-risk lesion with a concordancy of 98.6%(70/71)and the overestimation rate was 1.4%(1/71). The concordance of the histological type , calculated for 50 invasive carcinomas, was 92% (46/50) with a kappa value of 0.77.The concordance of the histological grade could be calculated for 38 invasive ductal carcinomas with the Elston-Elllis Method . It was 89.5% (34/38) with a kappa value of 0.57.@*Conclusions@#The pathology result of 14gUS-CNB is in good consistency with surgical excision for breast benign and malignant lesions.

Diagnostic value of ultrasound-guided 14 gauge-core needle biopsy in breast nodules / 中华普通外科杂志

Objective To analyze the diagnostic value of ultrasound guided 14 gauge coreneedle biopsy (US-CNB) in breast nodules.Methods We retrospectively analyzed the pathological results of US-CNB and surgical excision from 373 breast nodules in Peking University International Hospital from Sep 2016 to Nov 2018 to evaluate the accuracy of 14g US-CNB.Results A total of 349 patients (373 nodules) underwent US-CNB.US-CNB reported 282 benign lesions (75.6％,282/373),20 high-risklesions (5.4％,20/373),and 71 malignant lesions(19.0％,71/373).For 282 CNB reported benign lesions,the surgical pathology confirmed 235 lesions,46 for high-risk lesions and 1 for malignant lesion with a concordancy of 83.3％ (235/282)and the underestimation rate was 16.7％ (47/282).US-CNB identified 20 high-risk lesions.According to surgical results,15 were high-risk lesions and 5 were malignant lesions with a concordancy of 75％ (15/20) and the underestimation rate was 25％ (5/20).When it comes to malignant lesions,the excision results showed that 70 were malignant lesions and 1 was high-risk lesion with a concordancy of 98.6％ (70/71) and the overestimation rate was 1.4％ (1/71).The concordance of the histological type,calculated for 50 invasive carcinomas,was 92％ (46/50) with a kappa value of 0.77.The concordance of the histological grade could be calculated for 38 invasive ductal carcinomas with the Elston-Elllis Method.It was 89.5％ (34/38) with a kappa value of 0.57.Conclusions The pathology result of 14gUS-CNB is in good consistency with surgical excision for breast benign and malignant lesions.

Clinical effect of bone-anchored pendulum in treatment for class Ⅱ malocclusion / 中华医学美学美容杂志

Objective To study the clinical effect of bone-anchored pendulum in treatment for class Ⅱ malocclusion .Methods Twelve patients treated by bone-anchored pendulum were chosen .In each patient ,two microscrews were inserted in the anterior paramedian region of the median palatal suture ,6-9 mm posterior to the incisive foramen and 3 – 6 mm lateral to the median line .Pendulum that based on the microscrews was used to distalize maxillary first molar ,and the force applied was 250 g .Skeletal and dental changes were measured on cephalograms before (T1 ) and after (T2 ) distal-ization .Results Class Ⅰ molar relationship had been obtained 7 month after distalization .The aver-age distal movement of the maxillary first molars was 7 .07 mm ,and the inclination was 9 .93° .The second premolars were distalized an average of 5 .44 mm with inclinations of 5 .22° .The first premolars were distalized an average of 4 .09 mm with inclinations of 3 .95° .The maxillary anterior teeth were re-truded 0 .93 mm and palatally inclined 1 .66° .There was statistical difference in that change .Conclusions By the use of microscrew ,pendulum can distalize maxillary molar effectively without mesial move-ment of premolar and labial movement of incisor ,and no loss of dental anchorage is observed during the distal movement .

Expression pattern of FoxN1 gene and response to low temperature in Min pig / 中国兽医学报

Thymus is an important immune organ in mammals.FoxN1 plays an important role in regulating the development of thymic epithelial cells.Pig is a domestic animal of meat type,and is also a new kind of experimental animal model.In order to better understand the development characteristics of pig own immunity,the expression pattern of Minzhu FoxN1 in 2 month,4 month,6 month and 8 month thymus were detected by real-time PCR and Western-blot.At the same time,the changes of FoxN1 expression in Minzhu and Large white pig after cold stress were also detected.The results showed that FoxN1 expression level reached the highest level in 4 month,and declined in 6 month.This expression pattern is consistent with human and mouse.Cold stress could rise the expression level of Minzhu FoxN1,while have no effect on the Large white pig.

Effect of casein osphopeptide-amorphous calcium phosphate solution in preventing enamel demineralization / 南方医科大学学报

<p><b>OBJECTIVE</b>To evaluate the effects of casein osphopeptide-amorphous calcium phosphate (CPP-ACP) in preventing enamel demineralization.</p><p><b>METHODS</b>Enamel blocks were prepared from premolars extracted from orthodontic patients. The specimens were treated for 30 min daily for 7 days with one of the following agents: deionized water (negative control), CPP-ACP paste, and NaF solutions (positive controls) (0.2% and 2%). After the treatments, the specimens were immersed in a demineralizing solution (pH 4.5) for 7 days. The morphology and depth of the lesion were observed using scanning electron microscopy, and the content of Ca, P, Mg in the demineralized enamel was measured by electron probe micro-analyzer.</p><p><b>RESULTS</b>The demineralization rates in all the treatment groups were significantly smaller than those in the control group after 7 days of demineralization.</p><p><b>CONCLUSION</b>The application of CPP-ACP to the enamel surfaces can inhibit enamel demineralization with an equivalent effect to 0.2% NaF.</p>

Application of spiral computed tomography to evaluate the autogenous grafted bone in unilateral complete cleft lip and palate patients following early orthodontic tooth movement / 中华医学美学美容杂志

Objective The purpose of this study was to evaluate the outcome of autogenous bone grafts in unilateral cleft lip and palate patients following early orthodontic tooth movement,and to determine the volume of new bone formation in the bone grafted region with spiral computed tomography.Methods Computed tomography scans of 12 patients were taken immediately preoperatively and at 6 months postoperatively.The patients underwent bone grafting between 9 and 13 years of age were divided into two groups based on whether postoperative orthodontic tooth movement were initiated or not.Three-dimensional models were created in each period,and the defect of alveolar cleft and volume of the newly formed bone were calculated in each patient.The roots of the moved teeth and their positions to the alveolar bone were also observed.Results The preoperative cleft width and cleft volume were not significantly different between both groups.The volume of the newly formed bone in group A was (0.98±0.23) mm3,significantly higher than that in group B,which was (0.73± 0.15) mm3.The rate of newly formed bone in group A was (72.5 ± 11.9)％,significantly higher than that in group B,which was (53.2±9.7)％.The cleft adjacent teeth could move smoothly into the bone grated area,with no root resorption observed in the computed tomography scans.Conclusions Early orthodontic tooth movement can reduce bone resorption in autogenous bone grafted unilateral cleft lip and palate patients through the observation of spiral computed tomography.It plays an active role in the bone remolding process after bone grafting.

Clinical study on Yinzhi-huang combined with probiotics in the treatment of neonatal hyperbilirubinemia / 国际中医中药杂志

Objective To investigate the therapeutic efficacy of Yinzhi-huang combined with probiotics in the treatment of neonatal hyperbilirubinemia.Methods 80 neonates of hyperbilirubinemia were recruited into a treatment group (40 cases) and a control group (40 cases).The control group was treated with the gap blue light irradiation,besides,patients with rapid increase or high level of bilirubin were additionally treated with acid-correcting agents,Liver enzyme inducer,and albumin infusion; while the treatment group was additionally treated with Yinzhi-huang and probiotics on the basis of the control group.The values of serum total bilirubin before the treatment Was determined in both groups.During treatment,the changes of daily bilirubin,were monitored with percutaneous bilirubin monitor,and the time for serum bilirubin values decreased to normal level was studied in both groups.Results The two groups showed no significant difference in examination of the serum bilirubin values before treatment(P＞0.05).The daily bilirubin decreased value of treatment group(53.07± 17.80) μmol/L showed statistically significant compared with the control group(30.56± 13.43)μmol/L(P＜0.05); the time for serum bilirubin values decreased to normal level in the treatment group (4.87± 2.06) d was significantly different compared with the control group (7.12± 2.33) d,(P＜ 0.05).The apparent effective rate in the treatment group 75％ (30/40) was higher than that in the control group 35％(14/40)with statistical significance(x2=5.89,P＜0.05).The total effective rate in the treatment group 92.5％ (37/40)was higher than the control group 75％ (30/40)with statistical significance (x2=4.50,P＜0.05).Conclusion Yinzhi-huang combined with probiotics has better effects than conventional treatment in decreasing the daily bilirubin values,restoring normal serum bilirubin time of neonatal hyperbilirubinemia and it is worthy of clinical application.

STUDY ON THE ANTIFATIGUE EFFECT OF TEA CONTAINING ACANTHOPANAX SENTICOSUS / 营养学报

Objective: To study the antifatigue effect of tea containing Acanthopanax senticosus.Methods: Forty eight mice were randomized into 4 groups:three groups of tea containing Acanthopanax senticosus at doses of 50, 75, 100 ml/(kg?d), and the saline group.After 4 w, muscle and liver glycogen, serum LDH activity, BUN and blood LAC were measured.Results: Three tea groups showed increase of muscle and liver glycogen,and serum LDH activity, and decrease of serum BUN and LAC in swimming mice.The 75-100 ml/(kg?d) group was the best.Conclusion: Tea containing Acanthopanax senticosus has antifatigue effect.