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Objective:To evaluate the effect of down-regulating lncRNA LINC00263 targeting miR-4458 on the proliferation, migration, invasion and radiosensitivity of breast cancer SK-BR-3 cells.Methods:The expression differences of LINC00263 in breast cancer tissues, adjacent tissues, normal breast epithelial cells and breast cancer cells were determined by qRT-PCR. Transfection of LINC00263 shRNA in breast cancer SK-BR-3 cells down-regulated the expression of LINC00263, and the cloning experiment was used to detect the radiosensitivity. Breast cancer SK-BR-3 cells were treated with 6 Gy irradiation. CCK-8 assay was employed to detect cell proliferation. Flow cytometry was adopted to detect cell apoptosis. Transwell chamber test was performed to detect cell migration and invasion. Western blot was used to detect the expression levels of C-Caspase-3 and C-Caspase-9, MMP-2 and MMP-9 proteins. Bioinformatics software predicted that LINC00263 and miR-4458 had complementary binding sites, and the luciferase reporter system was utilized determine the targeting relationship between LINC00263 and miR-4458. LINC00263 shRNA and miR-4458 inhibitor were co-transfected into breast cancer SK-BR-3 cells, and 6 Gy irradiation was given to detect the changes in cell proliferation, apoptosis, invasion and migration.Results:The expression level of LINC00263 in breast cancer tissues was higher than that in adjacent tissues. The expression level of LINC00263 in breast cancer cells was higher compared with that in normal breast epithelial cells. The radiosensitivity of breast cancer SK-BR-3 cells was increased after transfection of LINC00263 shRNA. Transfection of LINC00263 shRNA and radiation exerted a synergistic effect, jointly inhibited breast cancer cell proliferation, migration and invasion, promoted cell apoptosis, up-regulated the expression levels of C-Caspase-3 and C-Caspase-9 proteins in cells, and down-regulated those of MMP-2 and MMP-9 proteins. Down-regulation of LINC00263 targetedly up-regulated miR-4458 expression. miR-4458 inhibitor reversed the inhibitory effect of LINC00263 shRNA combined with radiation on the proliferation, migration, invasion and apoptosis promotion of breast cancer SK-BR-3 cells.Conclusion:Down-regulating lncRNA LINC00263 targeting miR-4458 inhibits the proliferation, migration and invasion of breast cancer SK-BR-3 cells, and improves cell radiosensitivity.
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A 67-year-old female patient with incontinent dermatitis complicated with sacrococcygeal pressure ulcer was admitted to our unit in November 2017. The wound was treated with a new dressing based on the concept of wet healing, management of exudation, prevention of infection to promote wound healing. Meanwhile, the fecal incontinence was properly treated with colostomy bag combined with disposable negative pressure drainage device to avoid fecal contamination and aggravation of pressure ulcer. Incontinent dermatitis was treated with wound protective powder and wound protective film. After 14 days of treatment, the wound of pressure ulcer was reduced and the incontinent dermatitis was cured.
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Objective@#To explore the effects of application of Plato analysis combined with plan-do-check-action (PDCA) cycle in scientific research management of nurses with primary position title.@*Methods@#Twenty-six nurses with primary position title were recruited in this self pre- and post-control study. From January 2012 to December 2013, they were guided by normal scientific research management. In January 2014, the factors which hinder these nurses from writing papers were investigated by the questionnaire titled " the impediments that hinder nurses with primary position title from writing papers" . Based on " twenty-eighty percent laws" of Plato analysis method, the main influencing factors which hinder nurses with primary position title from writing papers were " had no idea" and " did not know how to write although with ideas" . From January 2014 to December 2015, based on the results of the survey and combined with PDCA cycle method, interventions were planned, carried out, analyzed by stage, and then improved continuously. Impediments which hinder writing papers of 26 nurses in January 2014 and December 2015, specific scientific research achievement, total scientific research achievement, and average scientific research achievement of these 26 nurses before intervention (from January 2012 to December 2013) and after intervention (from January 2014 to December 2015) were analyzed. Data were processed with McNemar exact probability test, Wilcoxon signed rank sum test.@*Results@#(1) The cumulative percentages of main impediments " had no idea" and " did not know how to write although with ideas" were decreased from 76.93% in January 2014 to 23.07% in December 2015. The number of nurses who " had no idea" was significantly reduced from 14 in January 2014 to 4 in December 2015 (P<0.01). The total number of nurses who had impediments which hinder writing papers was significantly reduced from 26 in January 2014 to 10 in December 2015 (P<0.01). (2) The total scientific research achievement and average scientific research achievement of the 26 nurses were significantly increased from 5 before intervention to 32 after intervention and 0.19 before intervention to 1.23 after intervention, respectively (Z=-4.838, -3.703, P<0.01). Among which, numbers of specific scientific research achievement of papers published in Journals of Statistic Source, papers published in other journals, science and technology awards, fund projects, national utility model patents, and patents for invention after intervention were all increased than those before intervention (P<0.05 or P<0.01).@*Conclusions@#Plato analysis can accurately analyze the main impediments that hinder nurses with primary position title from writing papers. Plato analysis combined with PDCA cycle can improve the overall ability of scientific research of nurses with primary position title and affect the achievements in nursing scientific research, which is worthy of promotion.
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Objectives To study the relationship of recurrent wheezing and obesity among infants. Methods In 118 recurrent wheezing infants, the growth level are assessed based on the WHO standard. Meanwhile, all the infants are divided into two subgroups (normal-weight, overweight/obese), and the tidal breath lung function (tidal breath flow volume loop) are analyzed. Results Among the infants of 1-3 years old, for the growth level, the recurrent wheezing infants have higher index of weight, but almost the same level of height, compared with the WHO standard. For the tidal breath lung function, tidal volume (VT/kg) is significantly impaired in overweight/obese subgroup in comparison to the normal-weight subgroup. But the ratio of time to peak tidal expiratory flow (tPF) to total expiratory time (tE) and ratio of volume to peak expiratory flow (VPF) to total expiratory volume (VE), which reflect the airway obstructions to a certain extent, have no statistical differences among the two subgroups. Conclusions Rather than the height index, the weight index is significantly higher in the recurrent wheezing infants than the level of WHO standard. Compared with normal-weight patients, the tidal volume of overweight/obese recurrent wheezing in overweight/obese group is lower, but no significant differences are found for other parameters of airway obstructions.