27-Hydroxycholesterol induces macrophage gene expression via LXR-dependent and -independent mechanisms

27-Hydroxycholesterol (27OHChol) exhibits agonistic activity for liver X receptors (LXRs). To determine roles of the LXR agonistic activity in macrophage gene expression, we investigated the effects of LXR inhibition on the 27OHChol-induced genes. Treatment of human THP-1 cells with GSK 2033, a potent cell-active LXR antagonist, results in complete inhibition in the transcription of LXR target genes (such as LXRα and ABCA1) induced by 27OHChol or a synthetic LXR ligand TO 901317. Whereas expression of CCL2 and CCL4 remains unaffected by GSK 2033, TNF-α expression is further induced and 27OHChol-induced CCL3 and CXCL8 genes are suppressed at both the transcriptional and protein translation levels in the presence of GSK 2033. This LXR antagonist downregulates transcript levels and surface expression of CD163 and CD206 and suppresses the transcription of CD14, CD80, and CD86 genes without downregulating their surface levels. GSK 2033 alone had no effect on the basal expression levels of the aforementioned genes. Collectively, these results indicate that LXR inhibition leads to differential regulation of 27-hydroxycholesterolinduced genes in macrophages. We propose that 27OHChol induces gene expression and modulates macrophage functions via LXR-dependent and -independent mechanisms.

27-Hydroxycholesterol induces macrophage gene expression via LXR-dependent and -independent mechanisms

27-Hydroxycholesterol (27OHChol) exhibits agonistic activity for liver X receptors (LXRs). To determine roles of the LXR agonistic activity in macrophage gene expression, we investigated the effects of LXR inhibition on the 27OHChol-induced genes. Treatment of human THP-1 cells with GSK 2033, a potent cell-active LXR antagonist, results in complete inhibition in the transcription of LXR target genes (such as LXRα and ABCA1) induced by 27OHChol or a synthetic LXR ligand TO 901317. Whereas expression of CCL2 and CCL4 remains unaffected by GSK 2033, TNF-α expression is further induced and 27OHChol-induced CCL3 and CXCL8 genes are suppressed at both the transcriptional and protein translation levels in the presence of GSK 2033. This LXR antagonist downregulates transcript levels and surface expression of CD163 and CD206 and suppresses the transcription of CD14, CD80, and CD86 genes without downregulating their surface levels. GSK 2033 alone had no effect on the basal expression levels of the aforementioned genes. Collectively, these results indicate that LXR inhibition leads to differential regulation of 27-hydroxycholesterolinduced genes in macrophages. We propose that 27OHChol induces gene expression and modulates macrophage functions via LXR-dependent and -independent mechanisms.

The Role of Lysophosphatidic Acid in Adult Stem Cells

Stem cells are undifferentiated multipotent precursor cells that are capable both of perpetuating themselves as stem cells (self-renewal) and of undergoing differentiation into one or more specialized types of cells. And these stem cells have been reported to reside within distinct anatomic locations termed “niches”. The long-term goals of stem cell biology range from an understanding of cell-lineage determination and tissue organization to cellular therapeutics for degenerative diseases. Stem cells maintain tissue function throughout an organism’s lifespan by replacing differentiated cells. To perform this function, stem cells provide a unique combination of multilineage developmental potential and the capacity to undergo self-renewing divisions. The loss of self-renewal capacity in stem cells underlies certain degenerative diseases and the aging process. This self-renewal regulation must balance the regenerative needs of tissues that persist throughout life. Recent evidence suggests lysophosphatidic acid (LPA) signaling pathway plays an important role in the regulation of a variety of stem cells. In this review, we summarize the evidence linking between LPA and stem cell regulation. The LPA-induced signaling pathway regulates the proliferation and survival of stem cells and progenitors, and thus are likely to play a role in the maintenance of stem cell population in the body. This lipid mediator regulatory system can be a novel potential therapeutics for stem cell maintenance.

Computed Tomography Diagnosis of Patent Ductus Arteriosus Endarteritis and Septic Pulmonary Embolism

No abstract available.

Hypoxia-dependent mitochondrial fission regulates endothelial progenitor cell migration, invasion, and tube formation

Tumor undergo uncontrolled, excessive proliferation leads to hypoxic microenvironment. To fulfill their demand for nutrient, and oxygen, tumor angiogenesis is required. Endothelial progenitor cells (EPCs) have been known to the main source of angiogenesis because of their potential to differentiation into endothelial cells. Therefore, understanding the mechanism of EPC-mediated angiogenesis in hypoxia is critical for development of cancer therapy. Recently, mitochondrial dynamics has emerged as a critical mechanism for cellular function and differentiation under hypoxic conditions. However, the role of mitochondrial dynamics in hypoxia-induced angiogenesis remains to be elucidated. In this study, we demonstrated that hypoxia-induced mitochondrial fission accelerates EPCs bioactivities. We first investigated the effect of hypoxia on EPC-mediated angiogenesis. Cell migration, invasion, and tube formation was significantly increased under hypoxic conditions; expression of EPC surface markers was unchanged. And mitochondrial fission was induced by hypoxia time-dependent manner. We found that hypoxia-induced mitochondrial fission was triggered by dynamin-related protein Drp1, specifically, phosphorylated DRP1 at Ser637, a suppression marker for mitochondrial fission, was impaired in hypoxia time-dependent manner. To confirm the role of DRP1 in EPC-mediated angiogenesis, we analyzed cell bioactivities using Mdivi-1, a selective DRP1 inhibitor, and DRP1 siRNA. DRP1 silencing or Mdivi-1 treatment dramatically reduced cell migration, invasion, and tube formation in EPCs, but the expression of EPC surface markers was unchanged. In conclusion, we uncovered a novel role of mitochondrial fission in hypoxia-induced angiogenesis. Therefore, we suggest that specific modulation of DRP1-mediated mitochondrial dynamics may be a potential therapeutic strategy in EPC-mediated tumor angiogenesis.

A Case of Hypoglossal Neurilemmoma in the Submandibular Space / 대한이비인후과학회지

Although neurilemmomas can arise from all types of cranial nerves except the optic and olfactory nerves, a hypoglossal neurilemmoma is extremely rare. Furthermore, since this neurilemmoma usually develops in the intracranial portion of the hypoglossal nerve, a hypoglossal neurilemmoma in the submandibular region is unfamiliar even to head and neck surgeons. However, the preoperative diagnosis of hypoglossal neurilemmoma in the submandibular region is very important because of the possibility of sacrificing the hypoglossal nerve during surgery under the incorrect impression of a salivary gland tumor. Therefore, we report a case of hypoglossal nerve neurilemmoma occurring in the submandibular area with a review of the literature focusing on preoperative differential diagnosis.

Effect of Menu Calorie Labels on Menu Sales and Consumer's Recognition at a Korean Restaurant in a Hotel / 대한지역사회영양학회지

Effect of Menu Calorie Labels on Menu Sales and Consumer's Recognition at a Korean Restaurant in a Hotel The role of calorie information is to help consumers make healthier food choices. However, calorie information is generally unavailable in restaurants. Even in high-end hotel restaurants, which try to provide high quality foods and service, calorie labeling is not mandatory. Therefore, the purpose of this study was to evaluate the effect of calorie labeling on menu sales and consumer's recognition at a Korean restaurant in Kangwonland hotel. The calorie contents of 10 dishes sold in the restaurant were calculated using the food composition table. After making a new menu plate displaying calorie information, the new menu plate and old menu plate were provided every other week for 4 weeks. When we compared the sales between the periods of calorie labeled and calorie unlabeled, sales of 4 items among the 5 food items providing less than 1000 kcal, increased, however the 3 items among the menu providing more than 1000 kcal decreased. As the survey results of total 405 consumers (male n = 232, female n = 173) showed the new menu plate, 68.2% of subjects recognized calorie labeling on the menu plate. Among the subjects who recognized calorie labeling, 58.3% answered that calorie information affected their food choices. And most of them answered that they chose lower calorie foods based on the information provided. The results suggest that displaying calorie information on the menu plate at a Korean restaurant was effective in changing consumer's food choices.

EFFECTS OF DIET PLUS EXERCISE ON VISCERAL FAT IN OBESE WOMEN: WITH SPECIAL REFERENCE TO THE INCREASE IN VO2max / 体力科学

The purpose of this study was to determine the loss of visceral fat during weight loss program with diet only or diet plus exercise in premenopausal obese women (age 44±6 yr) . One hundred seventeen women (body mass index 29±3 kg /m<SUP>2</SUP>) were divided into diet only group (DO, n=40) and diet plus exercise group (DE, n=77) . DE was further divided into two groups: a group with a small change in VO<SUB>2</SUB>max (DE<SUB>1</SUB>, n=26) and a group with a large change in VO<SUB>2</SUB>max (DE<SUB>2</SUB>, n=51) . Height, weight, fat mass, %fat, fat-free mass (FFM), abdominal total fat area (TFA), visceral fat area (VFA), subcutaneous fat area (SFA) and VO<SUB>2</SUB>max (ml/kg FFM/min) were measured before and after weight loss. The changes of weight, fat mass, %fat were significantly larger in DE than in DO. No difference was found in the changes of weight, fat mass, %fat between the DE<SUB>1</SUB> and DE<SUB>2</SUB>. Percentage of change in VFA was significantly larger in DE<SUB>2</SUB> (41±15%) than in DE<SUB>1</SUB> (31±16%) . These data suggest that both weight-loss programs (DO and DE) contribute to a remarkable decrease in visceral fat. Addition of exercise training, which would induce an improvement in VO<SUB>2</SUB>max, to dietary restriction, may elicit a greater effect on visceral fat.

Reliability and Validity of Questionnaire for Assessing Physical Workloads / 대한산업의학회지

OBJECTIVES: The aims of the study were to make a questionnaire for assessing physical workloads and to evaluate its reliability and validity. METHODS: A total of 220 workers (foundry workers 30, large vehicle assemblers 30, shipyard workers 75, and automobile manufacturers 80) completed a self-administered questionnaire and took examinations for physical work capacity and working heart rate. We excluded data with insufficient responses or incorrect physical work capacity and working heart rate. Finally, the data of 154 workers (70.0%) were used for our study. In order to evaluate the reliability and validity of the questionnaire, we used statistical analyses including the scaling assumption test and a comparison with the objective tool for physical workload which was evaluated by working energy expenditure. RESULTS: The items of the questionnaire in the same categories had close distribution in the evaluation of the scaling assumption. The item internal consistency was 0.41-0.73 for posture factor, and 0.62-0.79 for non-posture factor. The item discriminate validity was 100%. Cronbach's alphacoefficient of the total items was 0.73 (0.58 for posture factor and 0.74 for nonposture factor). In the correlation between working energy expenditure and questions, general physical activity (p=0.008), proportion of the workday with hands above shoulder (p=0.002), proportion of the workday with trunk bent (p=0.028), proportion of the workday with awkward posture (p=0.048), sweating after work (p=0.006), total scales (p=0.003) and Borg scale (p=0.011) all had statistical significance. CONCLUSIONS: Our questionnaire for assessing physical workloads demonstrated statistically significant reliability and validity. But the questions for the proportions of the workday with sitting work posture and with static posture should be modified via a larger study.