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1.
Infection and Chemotherapy ; : 107-114, 2003.
Artigo em Coreano | WPRIM | ID: wpr-722346

RESUMO

BACKGROUND: Small round structured virus (SRSV) is one of the common etiologic agents of viral gastroenteritis in childhood. However, SRSV has not easily been detected in many diarrheal outbreaks. Therefore, we experienced simultaneous outbreak of SRSV enteritis in two elementary schools, therefore, we analyzed the result of the surveillance. METHODS: We interviewed the pupil of two schools and questioned their demographic data, presence of gastrointestinal symptoms, contact with symptomatic persons within the last two weeks, and dietary history within the last one week in school. The specimens of the symptomatic pupil were collected for identification of bacteria (Shigella spp, Salmonella spp, E.coli O157 : H7) and viruses (Norwalk agent, SRSV, adenovirus, and astrovirus). RESULTS: The number of symptomatic patients was 193 (193/2843, 6.8%) and the distribution of symptom onset was unipolar. Frequent symptoms of the patients were abdominal pain (176/193, 91.2%), headache (111/193, 57.5%), vomiting (102/193, 52.8%), diarrhea (83/ 193, 43.0%), febrile sense (79/193, 40.9%), nausea (73/193, 37.8%), chilling (49/193, 25.4%), and tenesmus (8/193, 4.1%). We identified SRSV in 9 cases by PCR method, and analyzed the RNA polymerase gene by DNA sequencing; 2 genogroup (Genogroup I, II) and 3 genotype. CONCLUSION: We confirmed SRSV enteritis with different genotypes in two concurrent outbreaks. DNA sequencing and comparison of genotype among the isolated SRSV in near future are be necessary


Assuntos
Humanos , Dor Abdominal , Adenoviridae , Bactérias , Diarreia , Surtos de Doenças , DNA , RNA Polimerases Dirigidas por DNA , Enterite , Gastroenterite , Genótipo , Cefaleia , Náusea , Reação em Cadeia da Polimerase , Pupila , Salmonella , Análise de Sequência de DNA , Vômito
2.
Infection and Chemotherapy ; : 107-114, 2003.
Artigo em Coreano | WPRIM | ID: wpr-721841

RESUMO

BACKGROUND: Small round structured virus (SRSV) is one of the common etiologic agents of viral gastroenteritis in childhood. However, SRSV has not easily been detected in many diarrheal outbreaks. Therefore, we experienced simultaneous outbreak of SRSV enteritis in two elementary schools, therefore, we analyzed the result of the surveillance. METHODS: We interviewed the pupil of two schools and questioned their demographic data, presence of gastrointestinal symptoms, contact with symptomatic persons within the last two weeks, and dietary history within the last one week in school. The specimens of the symptomatic pupil were collected for identification of bacteria (Shigella spp, Salmonella spp, E.coli O157 : H7) and viruses (Norwalk agent, SRSV, adenovirus, and astrovirus). RESULTS: The number of symptomatic patients was 193 (193/2843, 6.8%) and the distribution of symptom onset was unipolar. Frequent symptoms of the patients were abdominal pain (176/193, 91.2%), headache (111/193, 57.5%), vomiting (102/193, 52.8%), diarrhea (83/ 193, 43.0%), febrile sense (79/193, 40.9%), nausea (73/193, 37.8%), chilling (49/193, 25.4%), and tenesmus (8/193, 4.1%). We identified SRSV in 9 cases by PCR method, and analyzed the RNA polymerase gene by DNA sequencing; 2 genogroup (Genogroup I, II) and 3 genotype. CONCLUSION: We confirmed SRSV enteritis with different genotypes in two concurrent outbreaks. DNA sequencing and comparison of genotype among the isolated SRSV in near future are be necessary


Assuntos
Humanos , Dor Abdominal , Adenoviridae , Bactérias , Diarreia , Surtos de Doenças , DNA , RNA Polimerases Dirigidas por DNA , Enterite , Gastroenterite , Genótipo , Cefaleia , Náusea , Reação em Cadeia da Polimerase , Pupila , Salmonella , Análise de Sequência de DNA , Vômito
3.
Korean Journal of Infectious Diseases ; : 123-128, 2000.
Artigo em Coreano | WPRIM | ID: wpr-119513

RESUMO

BACKGROUND: Rotavirus is the most common agent of acute gastroenteritis in childhood worldwide. Besides rotavirus, calicivirus is well known another important cause of acute gastroenteritis in childhood. However, caliciviral acute gastroenteritis has not been studied well in Korea. Here we report clinical manifestations of caliciviral acute gastroenteritis confirmed by RT-PCR. METHODS: Eleven patients who were admitted to Severance Hospital, Yonsei University College of Medicine from April 1998 to April 1999, were involved in this study. RNA was isolated from the stool of the patients. RT-PCR was done. Electrophoresis with the PCR products was done. Viruses were identified by electron microscope. Medical records were reviewed retrospectively. RESULTS: Infections occurred below 7 years of age in almost all patients. No sex predominance was found. The clinical manifestations were those of acute gastroenteritis, such as diarrhea, vomiting, poor oral intake, fever, nausea, or abdominal pain. Symptoms were less severe compared to rotaviral infection. CONCLUSION: In case of viral gastroenteritis caused not by rotavirus, we should keep in mind that calicivirus is a possible cause.


Assuntos
Humanos , Dor Abdominal , Diarreia , Eletroforese , Febre , Gastroenterite , Coreia (Geográfico) , Prontuários Médicos , Náusea , Reação em Cadeia da Polimerase , Estudos Retrospectivos , RNA , Rotavirus , Vômito
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