1.
J Biosci
;
2012 Dec; 37 (6): 971-977
Artigo
em Inglês
| IMSEAR
| ID: sea-161764
RESUMO
In this article, the traditional multi-site-directed mutagenesis method based on overlap extension PCR was improved specifically for complicated templates, such as genomic sequence or complementary DNA. This method was effectively applied for multi-site-directed mutagenesis directly from mouse genomic DNA, as well as for combination, deletion or insertion of DNA fragments.