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Southeast Asian J Trop Med Public Health ; 2000 Jun; 31(2): 394-8
Artigo em Inglês | IMSEAR | ID: sea-36210

RESUMO

Three serological tests (ELISA NOVUM, ELISA PU and ToxocaraCHEK) were compared using excretory-secretory (ES) antigen of second stage larvae of Toxocara canis on two sets of sera from patients aged 1-15 years, living in town and in the country, suspected of having larval toxocarosis. Of 60 serum samples examined for the presence of anti-Toxocara IgM and IgG antibodies 35 (58.3%) were positive with ELISA NOVUM, 30 (50.0%) with ELISA PU and 37 (61.6%) with ToxocaraCHEK. A significantly higher seropositivity with all the tests was detected in group of patients from the village (test chi2, p < 0.005) when compared with patients from the town. The highest sensitivity (100%) was detected for a rapid qualitative ToxocaraCHEK. Compared with this screening test, both ELISA sets showed higher specificity but a lower sensitivity. Consistent findings (+;-) with ELISA NOVUM and ELISA PU were 88.3%; ELISA NOVUM and ToxocaraCHEK 96.7% and ELISA PU and ToxocaraCHEK 86.7%. This comparison indicates the suitability of ToxocaraCHEK set for screening for its simplicity and rapidity in detecting anti-Toxocara antibodies.


Assuntos
Adolescente , Animais , Anticorpos Anti-Helmínticos/sangue , Antígenos de Helmintos/imunologia , Criança , Pré-Escolar , Reações Cruzadas , Ensaio de Imunoadsorção Enzimática , Proteínas de Helminto/imunologia , Humanos , Imunoglobulina G/sangue , Imunoglobulina M/sangue , Lactente , Kit de Reagentes para Diagnóstico , Sensibilidade e Especificidade , Toxocara/imunologia , Toxocara canis/imunologia , Toxocaríase/diagnóstico
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