Comparative stuy for LPS Extraction of NAG vibrio cholerae by EDTA, heating method and hot phenol method

Vibrio cholerae [NAG] was isolated from patients with diarrhea, identification by microbiological, biochemical and serological techniques, then Lip polysaccharide was extract from Vibrio cholerae [NAG][31G, 11G, 24G, 13G] by phenol and EDTA-heating method for comparison, The extracts were purified by Sepharose-4B gel. The results showed that EDTA-heating method was efficient and less expensive of time and material.