Busqueda de marcadores RAPD (Ramdom Amplified Polymorphic DNA) de dos especies Cripticas del complejo Sordida / Search of cripticas markers (Random Amplified Polymorphic DNA) of two species of the sordida complex

La erradicación de los insectos vectores Trypanosornci cruzt (agente etiológico del mal de Chagas) ha causado cambios inesperados en el comportamiento poblacional de éstos, reemplazando los vectores primarios por otros secundarios, como Triatoma sordida y T. guasayana (en Bolivia). Estos vectores han mostrado una alta capacidad de adaptación al hábitat humano. Por eso es importante identificar y estudiar la dinámica de poblaciones de estos insectos, sobre la base de marcadores de especie, una de las herramientas moleculares para este efecto propuesta en este trabajo es el RAPD. A partir de ADN extraido de especimenes de Triatoma guasayana, 7'. sordida Ql. T. sordida G2 y T. infestans, clasificadas anteriormente por isoenzimas; se amplificó fragmentos con 10 cebadores decaméricos del MT A (OPERON), 9 de los 10 cebadores utilizados presentaron perfiles RAPD, obteniéndose 170 perfiles de 1 a 8 fragmentos. Se pudo evidenciar la existencia de dos marcadores RAPD específicos: (1) un fragmento de 995 pb con el cebador OPAO1 para 7'. sordida G2, (2) un fragmento de 986 pb con el cebador OPAO2 para 7' sordida Gi

Nuclear rDNA-based molecular clock of the evolution of triatominae (Hemiptera: Reduviidae), vectors of Chagas disease

The evolutionary history and times of divergence of triatomine bug lineages are estimated from molecular clocks inferred from nucleotide sequences of the small subunit SSU (18S) and the second internal transcribed spacer (ITS-2) of the nuclear ribosomal DNA of these reduviids. The 18S rDNA molecular clock rate in Triatominae, and Prosorrhynchan Hemiptera in general, appears to be of 1.8 per cent per 100 million years (my). The ITS-2 molecular clock rate in Triatominae is estimated to be around 0.4-1 per cent per 1 my, indicating that ITS-2 evolves 23-55 times faster than 18S rDNA. Inferred chronological data about the evolution of Triatominae fit well with current hypotheses on their evolutionary histories, but suggest reconsideration of the current taxonomy of North American species complexes.

Quantitative phenetics and taxonomy of some phlebotomine taxa

Elucidating the evolution of Phlebotominae is important not only to revise their taxonomy, but also to help understand the origin of the genus Leishmania and its relationship with humans. Our study is a phenetic portrayal of this history based on the genetic relationships among some New Word and Old Word taxa. We used both multilocus enzyme electrophoresis and morphometry on 24 male specimens of the Old Word genus Phlebotomus (with three of its subgenera: Phlebotomus, Spelaeophlebotomus and Australophlebotomus), and on 67 male specimens of the three New World genera, Warileya, Brumptomyia and Lutzomyia, (with three subgenera of Lutzomyia: Lutzomyia, Oligodontomyia and Psychodopygus). Phenetic trees derived from both techniques were similar, but disclosed relationships that disagree with the present classification of sand flies. The need for a true evolutionary approach is stressed

Quantitative morphological evidence for incipient species within Lutzomyia quinquefer (Diptera: Psychodidae)

Morphological variation among geographic populations of the New World sand fly Lutzomyia quinquefer (Diptera, Phlebotominae) was analyzed and patterns detected that are probably associated with species emergence. This was achieved by examining the relationships of size and shape components of morphological attributes, and their correlation with geographic parameters. Quantitative and qualitative morphological characters are described, showing in both sexes differences among local populations from four Departments of Bolivia. Four arguments are then developed to reject the hypothesis of environment as the unique source of morphological variation: (1) the persistence of differences after removing the allometric consequences of size variation, (2) the association of local metric properties with meristic and qualitative attributes, rather than with altitude, (3) the positive and significant correlation between metric and geographic distances, and (4) the absence of a significant correlation between altitude and general-size of the insects

Triatominae as a model of morphological plasticity under ecological pressure

The use of biochemical and genetic characters to explore species or population relationships has been applied to taxonomic questions since the 60s. In responding to the central question of the evolutionary history of Triatominae, i.e. their monophyletic or polyphyletic origin, two important questions arise (i) to what extent is the morphologically-based classification valid for assessing phylogenetic relationships? and (ii) what are the main mechanisms underlying speciation in Triatominae? Phenetic and genetic studies so far developed suggest that speciation in Triatominae may be a rapid process mainly driven by ecological factors.

Changes in the Sexual Dimorphism of Triatominae in the Transition from Natural to Artificial Habitats

A shift from large to small average sizes was observed in Triatoma infestans and Rhodnius domesticus between field and domestic (or laboratory) conditions of life. It was more pronounced in the female specimens, leading to a subsequent reduction of sexual size dimorphism. This feature is discussed in terms of genetic and populational changes occurring from natural to artificial habitats, in particular those related to population densities. Sexual size dimorphism is then recommended as a new character to be used in the study of species of Triatominae adapting to domestic ecotopes

Baja probabilidad de transmisión de trypanosoma cruzi a humanos por triatoma sordida domiciliado en el departamento de Santa Cruz / Low probability of transmission od Trypanosoma cruzi to humans by triatoma sordid domiciled in the department of Santa Cruz

Entre más de 100 especies de triatominae (hemiptera: ruduviidae) descritas en el Nuevo Mundo, triatoma sordida es considerada de significancia epidemiológica como vector de trypanosoma cruzi por su amplia área de distribución y su tendencia para invadir ambientes domésticos.

Inter-relation of sylvatic and domestic transmission of Trypanosoma cruzi in areas with and without domestic vectorial transmission in Minas Gerais, Brazil

During the period 1980-1986, we captured triatomine bugs and mammalian reservoir hosts from sylvatic and domestic situations in different municipalities of the State of Minas Gerais. Trypanosoma cruzi was isolated from captured bugs, mammals and patients. After cultivation in LIT medium, the electrophoretic enzyme profiles were determined. We obtained atotal of 32 parasite isolates from regions with active domestic transmission, and 24 isolates form areas under control. For the first areas the results suggest introduction of T. cruzi from sylvatic habitats, through incursion of infected opossums and/or sylvatic T. sordida, which appears to have given rise to at least one acute human infection. Of particular interest is the finding of sylvatic opossums and a T. sordida nymph infected with ZB, that could indicate return of parasites from chronic human infections to sylvatic transmission cycles. For the areas under control we also interpret the results as interaction between sylvatic and domestic cycles of transmission, here through the invasion of houses by bugs carrying the Z1 zymodeme from the sylvatic environment. The Multivariate Correspondence Analysis gives a spatial description between the different parasite isolates and confirms the existence of a bridge in the opposite direction in the region with active vectorial transmission including the exporting of Z2 through the peridomestic environment into the sylvatic cycle. For the others areas this bridge corresponds especially to Panstrongylus megistus, importing Z1 into the domestic environment.

Transmision hereditaria de fenotipo "ojos rojos" en triatoma infestans (hemiptera:reduviidae) / Transmission hereditary of phenotype (red-eyes) in triatoma infestans (hemiptera:reduviidae)

Se ha realizado un analisis de transmision genetica del caracter ojo rojo en T.infestans recolectados en Tatarenda, Prov. Cordillera, Departamento Santa Cruz. Los datos obtenidos de las dos primeras generaciones nos hacen concluir que este caracter (o.r) es recesivo autoosomico, confirmando los trabajos de Wygodzinsky y Briones. No esta ligado alsexo como concluyeron Noe y Silva