value of electron microscopy in the study of sperm morphology in asthenospermia patients

Electron microscopic examination of 25 semen samples of asthenospermic patients was done. A wide variety of fine structural defects were observed in the majority of sperm tails in addition to head anomalies. Electron microscopy [EMy] demonstrated a great percentage of spermatozoal abnormalities [80 percent] when compared to those detected by the ordinary light microscope [50 percent]. Most of the sperm tails are abnormal and showed multiple defects including: confused arrangement of axoneme, extra coarse fiber, displaced or missing central pair and/or supernumerary doublets of axoneme. The result accentuates EMy as a greatly necessary tools in evaluating the fertilizing capacity of spermatozoa in male infertility

Comparative study of ultrastructural changes of the gingival hyperplasia induced by cyclosporine and dilantin

This study aimed to examine the gingival overgrowth induced by cyclosporine and dilantin ultrastructurally. Twenty human subjects were included, they were classified into three groups. From the results obtained, it was concluded that the ultrastructural changes in the hyperplastic gingival tissues were already present. In drug induced gingival enlargement related to cyclosporine and dilantin, the changes were mainly present in the connective tissue stroma. In cyclosporine affected patients, the connective tissue changes were fibrous in nature; but in dilantin, it was more cellular

Changes in the submandibular salivary glands and gingiva of the rat following chronic toxicity to thallium sulfate with special reference to hepatic afftection

Forty five adult male albino rats were divided into two groups. Group I composed of 3 rats and were treated with a daily oral dose equivalent to 1/20 LD50 of thallium sulfate for 10 weeks to study the effect of chronic toxicity to this rodenticide on the submandibular salivary gland, gingiva and liver. Group II composed of 15 rats and served as controls. Chronic toxicity to thallium sulfated in major histochemical and ultrastructural changes in the organs and tissues under investigation where there was a marked reduction in the amount of glycogen and protein while there was an increase in RNA and glycos aminoglycans, however the later was decreased in hepatocytes of the treated fats. Ultrastructurally the submandibular salivary gland secretory cells, x surface epithelium and fibroblasts of the lamina propria of the gingira as well as the hepatocytes presented toxic deposition of electron dense granules of thallium with marked reduction in the nuclear chromatin, number of mitochodria, Golgi bodies as well as the secretory granules, increase in the rough endoplasmic reticulum of low electron density was found. Hepatocytes presented collection of fat globules, abnormal figures of electron dense material and vacuolization. Dilatation of the bile canalicului and narrowing of blood sinusoids were also detected