RESUMO
Objective: To determine the effects of topical administration of 20% oltipraz solution on histomorphometrical and stereological aspects of skin tissue in full thickness skin wounds in laboratory rats
Methods: Thirty-six male Wistar portion rats [220 +/- 20 g] were randomly divided into three groups [n=12]. On the first day of experimentation, a 1-cm2 circular wound was made on the posterior surface of neck in all rats by removing a full thickness skin piece immediately after induction of anesthesia with ether inhalation. One group was treated with vehicle solution [DMSO alone]. The second group was treated daily with 20% oltipraz solution, and the third group, the control group, received no treatment. The wound closure rate was estimated our previously described method. The volume density of collagen bundles, vessels, and hair follicles, the vessels' length density, mean diameter of vessels and also fibroblast population were estimated by using stereological methods
Results: The oltipraz group indicated a significantly higher improvement [6.26% of the wound surface per day] than control and the vehicle treated groups [p=0.032]; furthermore, there was inconsiderable difference between the rate of wound closure in the group treated with vehicle [4.93% per day] and the control group [4.43% per day]
Conclusion: Oltipraz has positive influence on fibroblast proliferation and re-epithelization. A noticeable observation in our study was absence of scar formation in wounds which were treated by oltipraz and can be mentioned as an advantage of this drug
RESUMO
The residual shortening of limb is the major cause of gait disturbances in Legg-Calre-Perthes [LCP] disease. This study was a probing of limb length discrepancy after puberty by perfect method of teteroentgenogram in these patients. Residual shortening was measured by above method following completion of skeletal maturity in 46 patients and then the data were analyzed by computerized method. The patients were divided into three equal groups that they were treated by varus femoral osteotomy [FVO], abduction orthosis [AO] and untreated, respectively. The residual shortening was significantly more in AO group than FVO group and it was surprisingly a little more than the untreated group. The length of femur was identical in three groups [12.5, 11.1 and 10.4 millimeters, respectively]. The length of ipsilateral tibia was significantly different [2.8 mm shortening for AO group, 1.6 mm lengthening for FVO group and 1 mm lengthening for untreated group]. Overgrowth of ipsilateral tibia seems to be the leading cause of less limb discrepancy in FVO group and disuse atrophy seems to be the major reason for significant discrepancy in AO group which was somehow worse than untreated patients