Correlation of myeloid related proteins [MRP8/MRP14] and disease activity in juvenile rheumatoid arthritis

To evaluate the expression of myeloid related proteins MRP8/MRP14 in the serum and synovial fluid of JRA and their correlation with local and systemic parameters of disease activity. Thirty JRA patients [Group I], and ten controls [Group II] were included in the study. The patients were subjected to thorough history taking and clinical examination. Synovial fluid aspiration was done from ten JRA patients. Laporatory investigations included CBC, ESR, RE, ASO, ANA, CRP, ALT, urine analysis and synovial fluid analysis for white blood cell count [SF-WBCs], lymphocytes%, and acute phase serum amyloid. MRP8/MRP14 was assessed with ELISA technique in serum and synovial fluid samples. Serum level of MRP8/MRP14 was elevated in Group I in comparison to up II. The serum and synovial levels of MRP8/MRP14 in Group I showed no significant inter-correlation. The MRP8/MRP14 in group I showed significant positive correlation with ESR, CRP, DAS, and A-SAA. The SF MRP8/MRP14 in group I showed positive significant correlation with SF-WBCs and A-SAA. The elevated MRP8/MRP14 in the serum and synovial fluids of patients with JRA showed a significant correlation with local and systemic disease activity parameters. So, it can be used to monitor disease activity and patient's response to treatment

Relation of antimyeloperoxidase antibody t o rheumatoid arthritis disease pattern

Objective: To determine the presence of antimyloperoxidase [anti-MPO] in rheumatoid arthritis patients in order to assess its relation to disease pattern

Methodology: Serum antimyeloperoxidase antibodies were assessed with Enzyme Linked Immunosorbent Assay [ELISA] in 25 rheumatoid arthritis patients and 10 controls

Results: Anti-MPO was found in 60% of patients and 10% of controls with a high statistical difference between the patients and control groups [p > 0. 001]. Also there was a highly significant difference between the patients and controls regarding ESR [p < 0.001] and serum creatinine [p< 0.05]. There was no correlation between the mean anti-MPO and activity sore, duration of morning stuffiness and number of affected joints [p> 0.05]. There was a positive correlation between serum creatinine duration of RA, ESR and disease severity with mean of Anti-MPO among cases [p<0.01]

Conclusion: The use of Anti-MPO helps in the assessment of RA disease activity and help in the assessment of disease severity and prediction of renal affection. It cannot be used to predict the onset of RA but can be used as an indicator of long standing RA disease