Prevalence of nosocomial stenotrophomonas maltophilia infections in Assiut University Hospitals

Stenotrophomonas maltophilia is an emerging multidrug-resistant global opportunistic pathogen and is acquiring increasing importance as a nosocomial pathogen. This study aimed to determine the prevalence of nosocomial S. maltophilia infections and the important risk factors associated with such infections in Assiut University Hospitals. This study included 362 patients with nosocomial infections admitted to different wards and intensive care units [ICU] from March 2011 to March 2012. A total of 690 different clinical samples according to the site of infection were collected from them. The samples were processed and diagnosed by conventional bacteriological methods. A total of 35 strains of S. maltophilia were isolated from 362 patients [9.6%]. The commonest clinical manifestations were lower respiratory tract infections [71.43%], wound infections [17.14%], bacteraemia [8.57%] and urinary tract infections [2.86%]. The chest ICU showed the highest percentage of isolation [14.75%]. Previous antibiotic intake was found to be a significant risk factor for nosocomial Stenotrophomonas maltophilia infections. We conclude that nosocomial Stenotrophomonas maltophilia infections are significant in Assiut University Hospitals with lower respiratory tract infections being the commonest and previous antibiotic intake an important risk factor

Nosocomial acinetobacter infection in Assiut University Hospital

This study aimed to isolation of Acinetobacter spp, detect resistance patterns of isolated strains, phenotypic and genotypic detection of Metallo-beta-lactamase and typing of isolated strains from clinical and environmental respectively. this study includes 440 different clinical specimens, 672 environmental samples, inoculated on different culture media, confirmation of isolates by API20NE, PCR to detect 16SrRNA-23SrRNA gene, determine susceptibility pattern of isolates to different antibiotics and phenotypic and Genotypic detection of bla -OXA 51-like gene. 24 strains of Acinetobacter [5.45%] were isolated from 440 clinical samples, 27 strains of Acinetobacter were isolated from 672 environmental samples [4.017%]. Tetracycline was the most active drug against multi-drug resistant A.baumannii. [48/51 or 94%] of Acinetobacter isolates showed increase in zone of inhibition around IPM/EDTA disc compared with IPM disc alone. [49/51 or 96%] of Acinetobacter isolates were detected by presence of 16srRNA - 23 srRNA gene [universal gene present in all Acinetobacter species]. [49/51 or 96%] of isolated Acinetobacter spp showed band with blaOXA-51-like" genes. [37/51 or 72.5%] Acinetobacter isolates showed positive bands for class I integrase gene [gene responsible for multi drug resistance and outbreaks in hospitals]