Antibacterial property of leaf ethanol extracts of Persea americana Mill. variants against Staphylococcus aureus

Background@#Antibacterial resistance poses a significant health and economic burden worldwide. Relatedly, plant-based drug discovery remains an important adjunct to modern pharmaceutical research and development. Plants have an extensive record of being used as a form of alternative healthcare. For instance, the leaves of Persea americana Mill. (avocado) were used by traditional medicinal healers in Africa and the Philippines to alleviate common ailments such as skin ulcers and diarrhea. @*Objectives@#To determine their antibacterial activity, leaves of different P. americana variants maintained at the National Plant Genetic Resources Laboratory, University of the Philippines Los Baños were subjected to disk diffusion assay. @*Methodology@#Four P. americana leaf ethanol extracts (Cardinal, Morado, Semil 1, and Semil 2 variants) were tested in a range of concentrations (5 mg to 5 μg) against S. aureus (ATCC 25923) and E. coli (ATCC 25922) using standard disc diffusion. @*Results@#At 5 mg, all four P. americana leaf variants exhibited an inhibitory activity against S. aureus. Furthermore, Semil 2 variant showed the strongest relative antibacterial potential with activity at lower concentrations of 1.25 mg and 0.31 mg. On the other hand, all four variants did not suppress E. coli growth at the concentrations used. @*Conclusion@#The results indicate the potential antibacterial activity of P. americana leaf variants against S. aureus which is consistent with their ethnomedicinal use as a skin ulcer treatment since S. aureusis a common etiologic agent of skin ulcers. Furthermore, the findings suggest the four P. americana leaf variants, particularly Semil 2, as possible sources of novel antibacterial compounds against S. aureus.

COVID-19 vaccination knowledge, attitudes, risk perception, and intention among health workers in a district in Manila, Philippines

Background@#Vaccination is an effective public health measure. Health workers, who are at risk of acquiring infectious diseases due to their occupation, are important targets of vaccination. However, previous studies have shown varying rates of vaccine uptake among health workers. @*Objective@#This study aimed to determine the knowledge, attitudes, risk perceptions, and intention towards COVID-19 and vaccination of Filipino health workers in the city of Manila, Philippines. @*Methodology@#The study employed a sequential mixed methods design. A self-administered questionnaire was used to collect data on the study variables. Descriptive statistics was used to analyze the level of COVID-related knowledge, attitude, risk perception, and intention to receive the COVID-19 vaccine. Logistic regression was performed to identify the factors that were associated with intention. @*Results@#Majority (89.4%) had a good knowledge of COVID-19 but had little to moderate knowledge of COVID-19 vaccines. Around half reported feeling unsure about the safety and efficacy of the vaccines while the remaining half expressed intent to get the vaccine. Logistic regression analysis showed that the odds of intention to get vaccinated were higher among those with positive perceived self-efficacy, behavioral intention, external cues to action, perceived vaccine efficacy, and good knowledge on COVID-19 infection prevention. @*Conclusion@#Health workers play a crucial role in the promotion of public health measures such as vaccination to control the spread of COVID-19. The study confirms the need for strengthening education and communication strategies focusing on the safety and efficacy of COVID-19 vaccines to prevent the spread of false information and promote vaccine uptake among health workers.

Intersectoral collaborations for the prevention and control of Vector Borne diseases: A scoping review

Objectives@#This scoping review aimed to support a landscape analysis to identify lessons learned about intersectoral collaborations (ISCs) by describing their existing models in the context of dengue, malaria and yellow fever.@*Methods@#A scoping review following the methodology of Joanna Briggs Institute was performed using the following inclusion criteria: studies involving humans; studies discussing intersectoral collaborations, malaria/dengue/yellow fever, and prevention or control at any level; and studies in countries endemic for the aforementioned diseases. Studies were screened using Covidence, while data were extracted using NVivo.@*Results@#Of the 7,535 records retrieved, 69 were included in the qualitative analysis. Most ISCs were initiated by multilateral organizations and ministries of health, and none by communities. Strategies included advocacy, health education, research, public health measures, resource mobilization, service delivery and training; mostly employed on a community level. Monitoring and evaluation were mostly formative, ongoing, and participatory. Gaps included administrative and policy barriers, resource shortages, and inadequate research and training.@*Conclusions@#Multiple models of ISC exist in the literature. There is a need to develop a comprehensive framework for an effective and sustainable multisectoral approach for the prevention and control of VBDs ensuring adequate resources, active stakeholders, and strategies that span the entire socio-ecological spectrum.

Measuring stigma and discrimination towards people living with HIV among health care workers in a tertiary, government teaching hospital in the Philippines

@#<p style="text-align: justify;"><strong>BACKGROUND:</strong>The incidence of human immunodeficiency virus (HIV) infection in the Philippines is increasing. HIV-related stigma in the health care setting is a known barrier to healthcare access for people living with HIV (PLHIV).</p><p style="text-align: justify;"><strong>OBJECTIVE:</strong> The study aimed to identify stigmatizing attitudes and practices towards PLHIV among healthcare workers in Philippine General Hospital.</p><p style="text-align: justify;"><strong>METHODS:</strong> In this cross-sectional descriptive study, 375 healthcare workers were recruited via convenience sampling. A standardized questionnaire developed by the Health Policy Project was used.</p><p style="text-align: justify;"><strong>RESULTS: </strong>The study demonstrated concerns regarding transmission, particularly during drawing blood (87.1%),assisting in labor and delivery (82%), and dressing wounds (80.4%). Use of special infection-control measures (76.1%),wearing double gloves (72.8%), additional infection-control procedures during labor and delivery (72.2%), and wearing gloves during all aspects of patient care (70.2%) were reported as well. Perceptions such as the belief that pregnant women who are HIV positive must inform their families of their HIV status (82.1%), and that PLHIV engage in irresponsible behaviors (69.1%) and are promiscuous (66.4%) were also detected.</p><p style="text-align: justify;"><strong>CONCLUSION:</strong> The study confirmed the presence of HIV-related stigma among healthcare workers in Philippine General Hospital.This finding could potentially catalyze the development of stigma-reducing measures which could hopefully translate to improved healthcare for PLHIV.</p>