Avaliação farmacológica da detomidina no trato reprodutivo de éguas (Equus caballus)

A Detomidina é um agonista de receptores α2-adrenérgico, derivado imidazólico alcalóide, disponível sobre a fórmula de cloridrato, apresentarelação de seletividade entre receptor α2/α1de 260/1. O experimento foi conduzido para investigar a interferência da detomidina sobre o trato reprodutivo equino. Foram utilizadas vinte e cinco éguas sem raça definida (SRD), não prenhes, com idade entre 4 a 10 anos e peso de 350 a 450 Kg. As 25 éguas foram divididas de forma aleatória em 5 grupos, sendo que 3 grupos foram submetidos respectivamente a doses de 6, 9 e 12μg/kgde detomidina.Nos outros dois grupos, um deles foi aplicado Ioimbina em dose 0,10mg/Kg com antecedência de 20 minutos da aplicação de 6μg/kg de detomidina e o outro grupocontrole. Foram realizadas ultrassonográfias no modo B e Doppler do trato reprodutivo de éguas. O índice de pulsatilidade(IP), resistência (IR) e pulso arterial uterino direito (PAUD), bem como a avaliação subjetiva do útero e mesométrio de suprimento sanguíneo, não apresentaram diferençasignificativa(P0,0001) aos 10 minutos e 4,9±0,18mm (P>0,0001) aos 15 minutos, respectivamente...

The Detomidine is an agonist of α2-adrenergic receptors, derived imidazole alkaloid, available on the hydrochloride formula, is related to selectivity between receptor α2/α1of 260/1. The experiment was conducted to investigate the interference of detomidine on equine reproductive tract. Twenty-five mares were used mongrel, not pregnant, aged between 4-10 years old and weighing 350-450kg. The 25 mares were divided randomly into 5 groups, with 3 groups were submitted at doses of the detomidineof 6, 9 and 12μg/kg,respectively. In the other two groups, one applied yohimbine dose of 0.10 mg/kg 20 minutes prior to the application of 6μg/kg detomidine and in control group. All they were made sonographic evaluations in B mode and Doppler of the reproductive tract of mares. The pulsatility index (PI), resistance index (RI) and uterine blood right wrist (UBRW) and the subjective evaluation of the uterus and mesometriumblood supply, showed no significant difference (P0.0001) to 5 minutes, 2.7 ± 0.26 (P>0.0001) after 10 minutes and 4.9 ± 0.18 mm (P>0.0001) after 15 minutes, respectively...

The splenic follicle in human hepatosplenic schistosomiasis mansoni: A histological, immunohistochemical and morphometric study

Complex immune responses to Schistosoma mansoni infection, modulated by CD4+ and CD8+ T-Lymphocytes, underlie the different clinical presentations. In the peripheral blood from patieeeents with the hepatosplenic form the CD4+/CD8+ T-lymphocytes ratio is diminished. In order to investigate the participation of spleen in that immune response, the immunoarchitecture of the splenic follicules from 25 patients with hepatosplenic schistosomiasis and from four control individuals were studied by indirect immunoperoxidase technique and semiautomatic morphometry. The results showed that the folicular area (um²) was 17.2por cento greater in the schistosomatic group than in control individuals. The in situ CD4+/CD8+T-lymphocyte area ratio was reduced due to a significant increase of the expression of CD8+ suppressor lymphocytes. These results are similar to those described in peripheral blood and splenic homogenates, ad reinforce the importance of spleen in the modulation of the immune response observed in schistomomotic patients