RESUMO
The phytochemical investigation of the crude methanolic extracts roots and stem bark of Anthonotha cladantha (Harms) J.Léonard led to the isolation and identification of twelve secondary metabolites: 2,3-dihydroxypropyl hexacosanoate (1), hederagenine (2), cycloeucalenol (3), 2α-hydroxylupeol (4), betulinic acid (5), lupeol (6), heptacosan-2-one (7), triacontanoic acid (8), stigmast-4-en-3-one (9), β-sitosterol (10), stigmasterol (11), and stigmasterol-3-O-β-D-glucopyranoside (12). Their structures were elucidated with the help of their spectroscopic and physical data and by comparison with those reported in the literature. To the best of our knowledge, from all those compounds, 2,3-dihydroxypropyl hexacosanoate (1), hederagenine (2), cycloeucalenol (3), 2α-hydroxylupeol (4), and betulinic acid (5) are being reported for the first time from this genus. In addition, the acetylation of compound 1 afforded a new derivative 3-(hexacosanoyloxy)propane-1,2-diyl diacetate (1a).Compound 1 possessed a moderate α-glucosidase inhibitory activity with an IC50 value of 39.2 ± 0.22 µM; it neither showed antioxidant activity nor inhibition against the enzyme urease. Compound 1a exhibited weak antioxidant activity in the DPPH assay with an IC50 value of 80.3 ± 0.83 μM but was inactive against α-glucosidase and urease. Furthermore, both compounds 1 and 1a were inactive against seven pathogenic bacterial strains.
RESUMO
Introduction: Diabetes is a serious health problem; its prevalence is increasing in developing countries.This study aims to describe the socio-demographic, economic and health profile of diabetics attending the primary care units for the management of diabetes mellitus(DM). Methods: We carried out a cross-sectional study in 2019 on 257 diabetics in seven primary care units (Maendeleo, Funu, Uzima, CBCA-Nyamugo, 8thCepac-Buholo, Lumu and Charles-Mbogha).To collect the data, we used the survey questionnaire, medical tools and documents anthropometric tools and statement of the prices (for drugs, laboratory exams and public transport). Results: Most of respondents were female (79.4%), aged ≥ 45 years old (90.27%), with a level of study below secondary education (63.0), without paid employment (71.1 %) with a monthly income < $ 37.5 US (59.92%). Most of them had type-2 diabetes mellitus (93.3%). With family history (48.25%) and comorbidities (hypertension and stroke). All participants were on medication (77.0% on oral glucose-lowering drugs). Once a week glycemic control was assessed in six primary care units, we noted hyperglycemia in the majority of female 68.5% (OR = 2.25; P = 0.02); aged 55 or older 54.9% (OR = 2.62; P = 0.02), not respecting the diet 45.5% (OR = 2.09;P = 0.04) and ate the family meal on a common plate 40.5% (OR = 2.32; P = 0.007). Their monthly expenses covered the assessment of fasting blood sugar, medication, and food purchases. Body Mass Index has represented the increased (31.5% with overweight) and high (23.8% with obesity) disease risk. The waist circumference represented the abdominal obesity mainly in women. Conclusion: Diabetes is an economic and health burden for the patient, family and community. Primary care units lacked human, material and financial resources to care for diabetics