[ role of aqueous garlic extract [Allium sativum] on interleukin 10 and 12 in leishmania infected macrophages]

Leishmaniasis is a disease produced by several species of protozoa of the Leishmania genus. As a therapeutic agent, garlic [Allium sativum] is one of the most popular plants in traditional medicinal systems and is increasingly becoming important in herbal alternative therapy. The aim of the present study was to investigate the effects of aqueous garlic extract [AGE] on enhancing T-helper IL-10 and IL-12 cytokines in the culture via macrophages for engulfment and destruction of Leishmania. After proliferation of macrophages in culture and incubation with Leishmania during 72 hours, we added aqueaous garlic extract by several doses 9.25, 18.5, 37, 74 and 148 micro g/ml for 18, 24 and 48 hours. Trypan blue and MTT tests were carried out in order to test the safety of extracts and j744 cells treated with them, as well as cell viability. An enzyme-linked immunosorbant assay [ELISA] was performed on macrophages for interleukin IL-10 and IL-12. The results suggested that garlic extract may provide effective therapy against Leishmania. Our results showed that dose of 37 micro g/ml for 48 hours of garlic extract destructed promastigots by IL-12 secreted from macrophages. Based on the results of this study, IL-12 is crucial for defense against parasitic pathogens. IL-12 administration differentially affected immune response to invading Leishmania parasites

effect of aqueous garlic extract on interleukin-12 and 10 levels in Leishmania major [MRHO/IR/75/ER] infected macrophages

The aim of the present study was to investigate the immunomodulation effects of aqueous garlic extract [AGE] in the cultured macrophages infected by Leishmania major. After J774 macrophages proliferation in RPMI1640 and incubation with Leishmania for 72 hours, AGE was added in doses of 9.25, 18.5, 37, 74 and 148 mg/ml for 18, 24 and 48 hours and cell culture supernatants were harvested. The Leishmania infected J774 cells to assess the cell viability was examined using trypan blue and methylthiazol tetrazolium assay [MTT]. An enzyme-linked immunosorbent assay [ELISA] was performed on cell culture supernatants for measurement of interleukin IL-10 and IL-12. Dose of 37 mg/ml for 48 hours of garlic extract was the most potent dose for activation of amastigotes infected macrophages. In addition, AGE increased the level of IL-12 in Leishmania infected cell lines significantly. AGE treated cell is effective against parasitic pathogens, and AGE induced IL-12 differentially affected the immune response to invading Leishmania parasites