RESUMO
Tuberculosis [TB] is an important issue which its control is still unsatisfactory at global level. Traditional diagnostic techniques for active TB diagnosis are inadequate: the diagnostic gold standard is the culturel exam which suffers from lengthy processing and requires highly specialized laboratories. Nowadays more specifie tests hâve been recommended. The aim of this study is to evaluate the performance of Quanti FERON-TB [QFT] Gold In Tube-Test as a substitute for specifie test tuberculin skin test for diagnosis of latent tuberculosis infection in high risk groups. One hundred thirty four [134] individuels who worked in Bo-Ali hospital [Zahedan] enrolled in this study. They had no active tuberculosis. TST and QFT tests were performed. The cut-off point of TST was considered based on 15 [mm] or more indurations as positive. The resuit of QFT was evaluated by manufactured guidelines. Multivariate logistic regression was used to identify the putative risk factors of positive tests. Proportion of employees with latent TB were 1 1 1 [82.8%] were positive by either TST or QFT, and 76[56.7%] were positive by both tests. Agreement between the tests was high [73.8%, k=0.39; 95% E.21-0.44]. Positive family history of Tuberculosis was significant risk factor for both positive tests. This study showed high latent tuberculosis infection prevalence in hospital workers and high agreement between TST and QFT. Decision to select one of the tests will be depended on the population, purpose of study and availability of resources. The results revealed that the QFT can be appropriate alternative test for high risk group
RESUMO
Infections in accessory sex glands are considered as potential hazards to male fertility. These infections can affect different sites of the male reproductive tract such as the testis, epididymis and male accessory sex glands. Transmission of these infections to female partners causes genital infection, infertility and abortion. The aim of this study was to detect Mycoplasma, as one of the causatives, from the semen of infertile males using PCR assay. The survey considered 100 infertile men who referred to clinics and had not used antibiotics for 7 days prior to sampling. The infertility of cases was confirmed by a physician specialist. The sperm specimens were collected in sterile condition and sent to the laboratory rapidly. Specimens were examined for presence of Ureaplasma urealiticum and Mycoplasma hominis by PCR. Meanwhile, the history of vaginal infections and abortion in the female sexual partners was investigated. The results of multiplex PCR were compared with spermogram. All patients had no symptoms of genital infection. Of 100 infertile men, 33 [33%] were positive for CMU organisms [Chlamydia, Mycoplasma and Unreaplasma]. Uureaplasma urealiticum and Mycoplasma hominis were detected in 17 and 3 of patients respectively. Due to some problems in culturing of CMU organisms, PCR can be used as a diagnostic technique to detect such pathogens from seminal fluid of infertile men that leads to choose appropriate therapy in a shortest time