Molecular cloning and expression of EG95 gene of Iranian isolates of Echinococcus granulosus

Echinococcosis or hydatidosis is a chronic, zoonotic worldwide infection that occurs by the larval stages of taeniid cestodes of the genus Echinococcus. Iran is known as endemic region for this infection in the world. Vaccination has been considered as a good prevention method for this disease. Recombinant vaccines containing EG95 protein, against E. granulosus, has shown a high degree of protection against E. granulosus infection. In this study EG95 gene was extracted from Iranian isolates of E. granulosus and then cloned and expressed in expression vector. Protoscoleces were collected from sheep hydatid cysts. Then DNA and RNA were extracted from protoscoleces, and amplified by PCR and RT-PCR with specific primer. Afterward the purified RT-PCR products were successfully ligated into pTZ57R/T plasmid vector. The pcDNA3 plasmid was used as expression vector and Eg95 fragment sub cloned into this plasmid. The pcEG95 plasmid was digested by restriction enzymes to confirm cloning of this gene in pcDNA3 plasmid. In last step, the subcloned gene was expressed in CHO as eukaryotic cell. EG95 fragment successfully was subcloned in pcDNA3 and EG95 protein was expressed by eukaryotic cell. The recombinant EG95 protein was confirmed by SDS-PAGE and Western blot. Recombinant plasmid of pcEG95 was constructed successfully and express of recombinant EG95 protein was confirmed

study on giardiasis in pregnancy

In this study 404 pregnant women in Islamshahr, a district southwest of Tehran, were examined for giardiasis. Results showed 7.92% of them to be positive. Abdominal pain, nausea and diarrhea were observed as the most common symptoms in infected mothers. Results also indicated that hematological parameters and weight gain of infected pregnant women did not differ significantly from those without any parasitic infection. Also, birth weight, length and head circumference of the neonates of these two groups of mothers did not show any significant difference