[Molecular and histopathological findings on induced pulmonary lesions by bovine viral diarrhea virus]

The bovine viral diarrhea virus [BVDV] is one of the bovine respiratory system pathogens that represses lungs immunity and raises the pathogenicity of other bacteria and viruses. The aim of the present study was to evaluate the effect of BVDV in lung tissue. In this study, 30 bovine lung tissues were used. Histopathologic analysis showed different lesions in lung tissues. RT-PCR test results and histopathologic observations were studied for each sample and compared. Kai Square Test was used to study any possible association between the existence of BVD virus and incidence of four common lesions: 1- Interestitial pneumonia, 2- bronchitis and bronchiolitis, 3- edema, emphysema and atelectasis and 4- bronchopneumonia. If the assumptions of X2 test were not applicable, the Fisher Exact Test was used. The RT-PCR test for BVDV results was positive for 10 lung samples. 5 BVD positive cases were involved with interestitial pneumonia, while 10 BVD negative cases were not affected. Finally, in the studied lung tissues, only the 3 + degree or severe interstitial pneumonia was significantly associated with the existence of BVD virus. No significant relation was seen regarding other possible lesions, which may have resulted from the probable roles of bacteria or other viruses and toxic factors that were not studied in this project

[Clostridium perfringens type a related enteritis: a case report in dogs]

On June 2010, in Tehran province 7 out of 82 dogs in a kennel, showed clinical signs of enteritis which resulted in death [n=5]. Clinical findings were diarrhea, dysentery, fever, depression, neural sings and sudden death. Necropsy findings revealed hemorrhagic enteritis, catarrhal gasteritis, multifocal necrosis up to 0.5 cm in diameter in cerebral cortex with hyperemia and hemorrhage. Necropsy was done on 4 cases and intestinal contentes were cultured to characterize Clostridium perfringens type using PCR. Rapid test antibody for parvovirus and distemper virus showed that 2 out of 4 puppies were positive against distemper virus. According to nonsuppurative encephalitis, gliosis, intranuclear inclusion bodies in astrocytes, packed cell volume, interstitial pneumonia and positive reaction to distemper virus it can be concluded that primary distemper disease has been followed by, hemorrhagic enteritis and diarrhea as a secondary infection to Clostridium perfringens type A