[Morphological changes of the lacrimal gland and corneal epithelium after melatonin administration in white mice]

Study of the effects of melatonin on the lacrimal gland and corneal epithelium. In this study 20 white mice with the age of 11 months and weight of 20-23 g were divided equally in to the control and the experimental groups at random. The experimental group were daily injected intraperitoneally 10 mg/kg of melatonin dissolved in physiological serum for two weeks. The mice of control group were received only physiological serum. After the end of the injection period, the mice were killed with ether and their lacrimal glands and eyes were removed and processed using light microscope. Morphometric analysis was carried out to find the thickness of corneal epithelium, then, the data was evaluated with SPSS soft ware and T Test. The thickness of corneal epithelium in experimental group compared to control group was increased significantly [P<0.001] and different sizes of inclusions were observed in the lumen of acini. Melatonin has different effects on the lacrimal gland and corneal epithelium

[ effects of lead on the ultrastructure of uterine endometrium of immature Balb/c mice]

Study of the effects of lead on the ultrastructure of uterine endometrium. In this study 40 female Balb/c mice with the age of one week were divided into two control and experimental groups. The experimental group were daily injected intraperitoneally 75 microgram per gram of lead nitrate dissolved in physiological serum for two weeks. The mice of control group were received only physiological serum. After the end of the injection period, the mice were anesthetized and dissected, then their uterus were removed and divided into small pieces and transferred into karnovsky solution, After ward they were processed for electron microscope and prepared semithin and ultrathin sections, morphometry on electron micrographs were carried out by point counting and also the number of stromal cells in 50 fields of light microscope were counted too, the data was analyzed with SPSS software and student's T-Test. The endometrium epithelial cells in experimental group in comparison to control one had vacuoles inside the cytoplasm and volume fractions of nucleus to cell and mitochondria to cell were statistically different P<0.05 and also the mean number of the stromal cells had increased [P<0.05]. The entrance of the lead to the body from living environment in different ways can have adverse effects on the uterine endometrium

Morphometrie study of GnRH analogue, HMG and HCG effects on infrastructure of human endometrial glandular epithelium in early luteal phase

GnRH analogue, HMG and HCG administration are common protocol for ovulation induction in assisted reproductive technology [ART]. Since implantation rate in stimulated ART cycles is lower than unstimulated cycles and as endometrium plays an important role in embryo receptivity, effect of this protocol on the ultrastructure of human endometrial glandular epithelium was studied at LH 4 [embryo transfer time]. In this research endometrial biopsies were obtained from fertile women as well as infertile women who had undergone this protocol at LH 4. Quantitative and qualitative studies on endometrial glandular epithelium was performed by transmission electron microscopy [TEM] and morphometry and the results were statistically compared between the two groups. Qualitative results revealed presence of nuclear channel system [NCS], sub vacuole of glycogen and giant mitochondria [GM] in both groups. Similarly, in quantitative analysis, the volume fractions [Vv] of glycogen, mitochondria and rough endoplasmic reticulum to cell and also the Vv of euchromatin to nucleus were statistically not different [P> 0.05]. These results suggest that ovulation induction by GnRH analogue, HMG and HCG are not associated with advanced endometrial development and consequently, embryo transfer at this stage [before advanced endometrial development which occurs normally at LH 7 to LH 10] may cause a lower rate of implantation