RESUMO
This research aimed to investigate the viability of probiotic bacteria [Lactobacillus acidophilus LA-5 and Bifidobacterium lactisBB-12] and yogurt bacteria [Streptococcus thermophiles and Lactobacillus delbrueckii ssp. bulgaricus] in yogurt during the fermentation, immediately after fermentation and during refrigerated storage [21 d, 4°C]. Also the biochemical characteristics of milk as affected by the commercial 4-strain mixed starter culture were investigated. Storage time affected the viability of all bacterial species. The concentration of lactic acid during the fermentation increased in parallel with the titrable acidity, and the concentration of acetic acid was proportional to the viability of Bifidobacterium lactis. The acetaldehyde level was decreased in the yogurt from day 0 up to the end of the storage. Streptococcus thermophiles and Lactobacillus delbrueckii ssp. bulgaricuswere multiplied considerably during the fermentation. Streptococcus thermophiles could maintain its viability to the highest level, but Lactobacillus delbrueckii ssp. Bulgaricus lost its viability rapidly during the cold storage compared to Streptococcus thermophilus. The multiplication and viability of probiotic bacteria were also influenced by the associative strains and species of yogurt organisms. Bifidobacteria counts were satisfactory. The loss of viability for bifidobacteria was gradual and steady during the storage, and they showed good stability during the storage as compared to Lactobacillus acidophilus