[Clinical and histopathological evaluations of local honey application in the healing of experimental wounds in dog]

Honey has been generally used in traditional medicine for healing different kind of wounds. In this research complex, the clinical and histopathological effects of West Azarbayjan non-boiled honey in healing process of surgical wounds [cuts] were evaluated. This study was performed to evaluate the effect of honey on healing time and duration of wound closing in dog. 30 dogs, each weight was almost 25kg, were divided into 2 clinical [n=10] and histopathological [n=20] groups. In each group, after surgical preparation and anesthesia surgical wounds were produced by a similar pattern and size [rectangle; 25×50 mm[2]] in the thoracolumbar region. Wounds on the left side arbitrary taken as treatment group and on the right side as control group. Postoperative treatment in the honey group was included daily wound irrigation with normal saline and then topical application of 20g honey. In the control group only irrigation with normal saline was performed. Clinical and histopathological parameters were evaluated in each group 7, 14, 21 and 28 days after operation. Honey caused in acceleration an healing and surgical wound closure and in decreasing the level of infection and secretion in the experiment group in compare to control group which was more significant at days 14 and 28 [p<0.05]. Decreasing the area of wound during experiment in honey treated group was 11.1% more than control group. From histopathological aspect, especially from the second week on, less neutrophilic chemotaxis was found in treatment group than in the control group and in treatment group the compact connective tissue was developed more rapidly. West Azarbayjan non-boiled honey is potentially effective in surgical wound healing

Effect of a static magnetic field on bone healing in the dog: radiographic and histopathological studies

Although the promotional effects on bone healing of pulsed electromagnetic fields [PEMF] have been well demonstrated, the effects of static magnetic fields [SMF] remained unclear. In this study, effects of a custom-made magnetic wrap on radiographic and histopathological aspects of bone healing using a canine unstable osteotomy gap model were investigated. After an osteotomy of the midshaft radius, bone healing was evaluated over an 8-week-period in the control dogs [n = 5] and dogs exposed to SMF [1000 gauss] [n = 5]. Bone healing was evaluated by qualitative and quantitative evaluation of serial radiographs every 2 weeks. Histopathological study was also performed on osteotomized radii upon completion of the experimental period. Dogs exposed to SMF had more advanced radiographic healing of osteotomy sites. Cellular morphology scores of the SMF group was significantly greater as compared with the control group [P<0.05]. These results suggested that using the osteotomy gap model, SMF enhanced the radiographic and histopathological aspects of bone healing in dogs. Dogs at risk for delayed healing of fractures may benefit from treatment with SMF

Evaluation of topical application of honey in prevention of post-operative peritoneal adhesion formation in dogs

The objective of this study was to determine the effectiveness of topical administration of honey in prevention of or reduction in post-surgical peritoneal adhesions in dog. The study was conducted on 18 adult female mixed-breed dogs with a mean +/- SD weight of 20 +/- 4.25 kg. A standardized surgical trauma [serosal trauma model] was made in the dogs' descending colon to induce adhesion formation. The control group [n = 9] received saline treatment. In the experiment group [n = 9], the lesions were covered with honey. Three weeks after laparotomy, the dogs were sacrificed and the adhesions were graded according to the Evans's scoring system. Overall, 67% [n = 6] of the control and 22% [n = 2] of the experiment animals presented with moderate [grade-2] and severe [grade-3] adhesions. In the control group, there were 3 [33%] dogs with grade-0 or grade-1 adhesions, while in the experiment group there were 7 [78%] dogs with the same grades of adhesions. The difference between the two groups was significant [P<0.05]. It was concluded that honey is a useful covering for damaged peritoneal surfaces and has high effectiveness in reducing post-operative adhesions

[Evaluation of the effect of clomiphen citrate on osteoporosis in the dog model: biomechanical and histopathological studies]

To evaluate the short term effect of clomiphen citrate on osteoporosis in dog. Experimental study. Ten dogs of both sexes with average weight of 23 +/- 5.6 Kg, were randomly assigned to either control [n=5] or experimental groups [n=5]. Osteoporosis was induced by immobilizing the right hindlimb by means of fiberglass cast. Five dogs received clomiphen citrate [5 mg/kg per os] once a day for 42 days. All dogs were euthanized on day 42 and tibias were harvested. Bone biomechanical properties and microscopic structure of casted and uncasted tibias were studied. Independent t-test was done for biomechemical values. Significant differences in the values of examined mechanical properties were found between controt and clomiphen - treated dogs. [p<0.05] No remarkable histopathological changes were detected in the tibias of casted limb in Clomiphen-treated dogs. These findings indicate that short term clomiphen therapy may have promising effects on prevention of osteoporosis in dog

Detection and characterization of verotoxin-producing strains among sorbitol non-fermenting Escherichia coli

The presence of genes for verotoxin 1 and 2 [VT1 and 2] among sorbitol non-fermenting Escherichia coli isolates from diarrhoeal cases was assessed using polymerase chain reaction assay. Of 60 [88%] positive isolates, 37 [62%] harboured VT1 and 23 [38%] both VT1 and VT2. In HeLa cell adherence assay, 48 [71%] isolates exhibited mannose-resistant adherence to HeLa cells. Multidrug resistance was observed in 56 [82%] isolates, with ampicillin, chloramphenicol, streptomycin, sulfamethoxazole-trimethoprim and tetracycline pattern being the most common. There were 13 common and 22 single biochemical phenotypes identified. Isolates belonging to common biochemical phenotypes normally had a similar pattern of adherence and VT production, but differed greatly in their pattern of antibiotic resistance, pointing to a high rate of antibiotic-resistance transfer among these isolates