RESUMO
Background: Papaver bracteatum Lindl. Commonly known as Iranian poppy is an important medicinal plant due to the presence of benzylisoquinoline alkaloids
Objective: To evaluate the hairy root culture as a novel method for thebaine production
Methods: To optimize the hairy root culture of P. bracteatum, five strains of Agrobacterium rhizogenes [ATCC15834, C318, A13, 9453 and A4] were used by means of Sonication-Assisted Agrobacterium-mediated Transformation [SAAT]
Results: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate [28.5 %] was observed 4 weeks after inoculation, once the explants were subjected to sonication [45 s] following a heat treatment of 41 degree C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers
Conclusion: Strain C318 proved to be more efficient than the other strains to hairy root induction ability. Among different experimental conditions, the highest transformation rate [28.5 %] was observed 4 weeks after inoculation, once the explants were subjected to sonication [45 s] following a heat treatment of 41 degree C for 5 min. Transgenic status of the hairy roots was confirmed by PCR using rolB specific primers
RESUMO
Recently, the production of biosurfactants in bioreactors and their use in various pharmaceutical, chemical and food industries have been developed. Optimum production is directly related to the physicochemical condition of culture medium [such as pH and temperature] and engineering parameters of bioreactors [such as aeration rate, volume of operation and the amount of energy input]. Understanding the gas transfer in shaken bioreactors equipped with a sterile closure is advantageous to avoid oxygen limitation or carbon dioxide inhibition of a microbial culture. In this study, the effect of aeration rates [due to using different design closures] on the amount of biosurfactin production by Bacillus subtilis ATCC 6633 in a ventilation flask as a miniaturized bioreactor was investigated. The highest biosurfactin concentration [0.0485 g/L/h] was obtained in the optimum conditions in which the amount of filling volume and shaking frequency were 15 ml and 300 rpm, respectively. The specific aeration rate [q[in]] and maximum oxygen transfer rate [OTR[max]], were calculated 1.88 vvm and 0.01 mol/L/h, respectively. The results showed the significant biosurfactin productivity increase under non-oxygen limiting condition