DNA-feulgen cytophotometry in hyperplasia and adenocarcinoma of the endometrium

The present study was carried out on 35 endometrial hyperplasia, 30 endometrial carcinoma and 5 normal proliferative phase endometrium. Cases were stained with hematoxylin and eosin and Feulgen stain for cytophotometry. The majority of cells in the normal and the hyperplastic groups, including atypical hyperplasia were within the diploid range, whereas more than 65% of the cells in the carcinoma group, were aneuploid with increase in number of aneuploid cells as the grade of the tumor increased. The number of aneuploid cells was also proportionate to the nuclear area measurements. Small nuclear areas were usually diploid, while larger ones were associated with more DNA and were aneuploid content. Such results indicated that cytophotometry may be a helpful adjuvant in routine histolopathological diagnosis, increasing the diagnostic accuracy and shedding light on the biological potential of the malignant cells

Histochemical study of epithelial mucin in endometrial hyperplasia and adenocarcinomas

The pattern and type of mucin were studied on 37 curettage specimens. These cases were formed of 25 endometria showing normal proliferative and secretory phase and 12 endometria showing hyperplastic changes. Fifteen hysterectomy specimens from cases of endometrial adenocarcinoma were also examined in this work. All cases were stained by Hematoxylin and Eosin, periodic acid-schiff [P.A.S.] and alcian blue. The cases of endometrial hyperplasia showed the same distribution of both types of mucin secreted by the normal endometrial glands, although increased in quantity. Most of endometrial adenocarcinomas contained different quantities of cellular mucin, which was found to be more abundant and widely distributed in the well differentiated than in the moderately or poorly differentiated ones. It was observed that the histochemical properties, site and pattern of mucin secretion in these cases strongly resembled that seen in the normal endometrium. This may play a role in differentiating adenocarcinoma of endometrial origin from other carcinomas in the female genital tract like that of endocervical origin

histopathological study of grade associated angiogenic responses in mammary carcinoma

65 Radical mastectomy specimens were sent to the Pathology Department of Kasr El Einy Hospital and 10 postmortem breast samples were subjected to routine light microscopy, to determine the histological type and the grade of the tumour. This was followed by determining the degree of angiogenesis in the tissues examined using a graduated eye piece [point counting method] and the degrees of neovascularization were divided into minimal < 15 percent of total surface area, moderate 15-55 percent and marked > 55 percent SA. It was apparent from the results that as the tumour grade increased there was a proportionate increase in host tissue neovascularization, it thus may be assumed that the actual degree of neovascularization possibly enhances tumour cell aggressiveness and hence the histological grade increases. Another finding of some importance was that duct carcinomas appeared to be more likely to produce pronounced angiogenic responses in the tissues than lobular types; this may in part explain the more aggressive behaviour of the duct carcinoma group compared to the lobular neoplasms