RESUMO
OBJECTIVE@#To verify the effect of the mutant gene vps4b on the expression of tooth development-related proteins, dentin sialophosphoprotein (DSPP) and collagenⅠ (COL-Ⅰ).@*METHODS@#Paraffin tissue sections of the first molar tooth germ were obtained from the heads of fetal mice at the embryonic stages of 13.5, 14.5, and 16.5 days and from the mandibles of larvae aged 2.5 and 7 days after birth. The immunohistochemical method was used to detect the expression and location of DSPP and COL-Ⅰ in wild-type mouse and vps4b knockout mouse.@*RESULTS@#DSPP and COL-Ⅰ were not found in the bud and cap stages of wild-type mouse molar germ. In the bell stage, DSPP was positively expressed in the inner enamel epithelium and dental papilla, whereas COL-Ⅰ was strongly expressed in the dental papilla and dental follicle. During the secretory and mineralized periods, DSPP and COL-Ⅰ were intensely observed in ameloblasts, odontoblasts, and dental follicles, but COL-Ⅰ was also expressed in the dental papilla. After vps4b gene knockout, DSPP was not expressed in the dental papilla of the bell stage and in the dental papilla and dental follicle of the secretory phase. The expression position of COL-Ⅰ in the bell and mineralization phase was consistent with that in the wild-type mice. Moreover, the expression of COL-Ⅰ in the dental papilla changed in the secretory stage.@*CONCLUSIONS@#Gene vps4b plays a significant role in the development of tooth germ. The expression of DSPP and COL-Ⅰ may be controlled by gene vps4b and regulates the development of tooth dentin and cementum together with vps4b.
Assuntos
Animais , Camundongos , ATPases Associadas a Diversas Atividades Celulares , Genética , Colágeno , Metabolismo , Complexos Endossomais de Distribuição Requeridos para Transporte , Genética , Proteínas da Matriz Extracelular , Metabolismo , Camundongos Knockout , Dente Molar , Odontoblastos , Fosfoproteínas , Metabolismo , Sialoglicoproteínas , Metabolismo , Germe de DenteRESUMO
Objective To study the effect of Kangfuxin Liquid combined with lidocaine assisted oxygen therapy on the treatment of oral ulcer after radiotherapy of nasopharyngeal carcinoma. Methods 64 cases of nasopharyngeal carcinoma after radiotherapy of oral ulcer from July 2015 to July 2017 were divided into observation group (n=32) and control group (n=32). The control group were given oxygen therapy and lidocaine hydrochloride gel,and the observation group were given Kangfuxin liquid based on the treatment of control group. Results The total effective rate of observation group was 93.75%, higher than 71.88% in the control group (P<0.05). After treatment, patients with T lymphocyte subsets level indicators improved significantly, the observation group was increased more than control group (P<0.05). The healing time of the observation group [(3.27±0.98)d] was lower than the control group [(5.11±0.84)d], the difference was statistically significant between two groups (P<0.05). Conclusion Kangfuxin liquid combined with lidocaine assisted oxygen therapy treated in patients with nasopharyngeal carcinoma after radiotherapy get good efficacy.
RESUMO
Objective To study the effect of Kangfuxin Liquid combined with lidocaine assisted oxygen therapy on the treatment of oral ulcer after radiotherapy of nasopharyngeal carcinoma. Methods 64 cases of nasopharyngeal carcinoma after radiotherapy of oral ulcer from July 2015 to July 2017 were divided into observation group (n=32) and control group (n=32). The control group were given oxygen therapy and lidocaine hydrochloride gel,and the observation group were given Kangfuxin liquid based on the treatment of control group. Results The total effective rate of observation group was 93.75%, higher than 71.88% in the control group (P<0.05). After treatment, patients with T lymphocyte subsets level indicators improved significantly, the observation group was increased more than control group (P<0.05). The healing time of the observation group [(3.27±0.98)d] was lower than the control group [(5.11±0.84)d], the difference was statistically significant between two groups (P<0.05). Conclusion Kangfuxin liquid combined with lidocaine assisted oxygen therapy treated in patients with nasopharyngeal carcinoma after radiotherapy get good efficacy.