RESUMO
Objective: The aim of the study is to detect the frequency ofA2B blood groups among the AB donors.Method: This cross-sectional observational study which wascarried out at Transfusion Medicine department, BangabandhuSheikh Mujib Medical University, Dhaka from January 2018 toJuly 2018 over 142 subjects. After selection of healthy donorsblood group was detected by standardized tube technique.Anti- A1 lectin study was done for all blood donors with bloodgroup AB. Based on the serological reactivity, blood group wasclassified into A2B and Rh-D negative or positive.Results: In the study, it was found that among 142 AB bloodgroup donors, majority blood donors were male 130 (91.5%)and rest were female 12 (8.5%). Among 10 A2B blood groupdonors 25% were female and 5.38% were male. More than halfdonors 78 (54.9%) belonged to age 21-30 years. The meanage was 27.68 SD±6.76 years with ranged from 18 to 50years. It was observed that 132 (93.0%) belonged to A1Bsubgroup and 10 (7.0%) belonged to A2B subgroup. The studyalso revealed that 126 (95.5%) A1B individuals were Rhpositive, 6 (4.5%) A1B individuals were Rh-negative. All A2Bindividuals were found Rh-positive.Conclusion: From our study we found that, the frequency ofA2B among the female donors is more than four times higherthan the male donors. As the A2B is the rare subgroup but thefrequency of this subgroup in this study recommend the extraattention for the physician and staff working in the TransfusionMedicine department.
RESUMO
Cancerous inhibitor of protein phosphatase 2A (CIP2A) has been identified as one of the most commonly altered proteins in humancancers. It blocks the tumor-suppressive action of protein phosphatase 2A (PP2A) complex and enhances malignancy. Thirty-fivepatients with squamous cell carcinoma of the oral cavity underwent surgical resection of the tumor. CIP2A was assessed by quantitativereal-time PCR in the resected tumor tissues and in their adjacent normal tissues. CIP2A was found to be overexpressed inall oral squamous cell carcinoma (OSCC) specimens in comparison to their surrounding normal tissue. CIP2A overexpression wasstatistically correlated with poor prognostic feature of the tumor. Thus, a high expression level of CIP2A was associated with shortersurvival. In conclusion, CIP2A is upregulated in OSCC, and its overexpression is correlated with aggressiveness of the tumor andpoor outcome and survival. It may serve as a prognostic marker of OSCC.
RESUMO
Helicobacter pylori is a Gram-negative bacteria that is responsible for gastritis in human. Its spiral flagellated body helps in locomotion and colonization in the host environment. It is capable of living in the highly acidic environment of the stomach with the help of acid adaptive genes. The genome of H. pylori 26695 strain contains 1,555 coding genes that encode 1,445 proteins. Out of these, 340 proteins are characterized as hypothetical proteins (HP). This study involves extensive analysis of the HPs using an established pipeline which comprises various bioinformatics tools and databases to find out probable functions of the HPs and identification of virulence factors. After extensive analysis of all the 340 HPs, we found that 104 HPs are showing characteristic similarities with the proteins with known functions. Thus, on the basis of such similarities, we assigned probable functions to 104 HPs with high confidence and precision. All the predicted HPs contain representative members of diverse functional classes of proteins such as enzymes, transporters, binding proteins, regulatory proteins, proteins involved in cellular processes and other proteins with miscellaneous functions. Therefore, we classified 104 HPs into aforementioned functional groups. During the virulence factors analysis of the HPs, we found 11 HPs are showing significant virulence. The identification of virulence proteins with the help their predicted functions may pave the way for drug target estimation and development of effective drug to counter the activity of that protein.