RESUMO
Background: Among new therapies emerging in the medical field, the use of platelet-rich plasma (PRP) in human reproduction has not yet been explored. Platelet-rich plasma (PRP) has a potential effect on tissue repair through proliferation and differentiation of tissue progenitor cells. The aim of this study was to evaluate the effect of PRP on the testis structure and function in the rabbit model. Material and methods: A total of 30 male rabbits were recruited in this study. They were allocated into two groups (15 in each group) to receive an injection of PRP (PRP Group), or normal saline (Control Group) Results: there were statistically significant differences in Means of germinal layer width, Leydig cell number, and Sertoli cell number was significantly higher in the PRP group compared to that in the control group ( P < 0.05). The PRP group had a higher means of sperm concentration and normal morphology compared with the control groups (P < 0.05). Conclusion: the platelet-rich plasma is found to have a good potential effect on the testicular tissue that improved the histological and functional aspects and could be considered a promising future treatment for hypogonadism status in many disorders.
RESUMO
The choroid plexus is composed of highly specialized vascularized epithelial tissues present in 4 ventricles of the brain. The central core of the choroid plexus is occupied by blood vessels. The choroid plexus has many functions like CSF production and synthesis of bioactive peptides. Antigen like Proliferating cell nuclear antigen PCNA, a 36 kd DNA polymerase delta auxiliary protein; is involved in the proliferation of neoplastic and non-neoplastic cells. PCNA has a role in DNA synthesis and repair. Previous studies reported that the PCNA expression in the cytoplasm of cells can be demonstrated by histochemical stains. Total 50 adult male New Zealand rabbits (Oryctolagus cuniculus) were sacrificed, a sample of lateral and 4th ventricles was prepared for H and E and immunohistochemical stains for PCNA. PCNA expression was more in lateral ventricle than 4th one indicates the higher involvement in biological function. The higher PCNA expression in the choroidal cells of the lateral ventricle suggests the clinical importance and pharmacological role in many neurological diseases. Significance Statement: The study is conducted to study the differences in choroid plexus of the ventricles separately, and to see the clinical importance for that, and also to evaluate the PCNA agent expression in lateral and 4th ventricles.