Methoxyamine enhances 5-fluorouracil-induced radiosensitization in colon cancer cell line HT29

Objective: This study intended to observe the effects of methoxyamine [Mx] on cytotoxic effects and DNA damage caused by 5-Fluorouracil [5-FU] in combination with gamma radiation in a human colon cancer cell line, HT29

Materials and Methods: In this experimental study, HT29 cells were cultured as a monolayer and treated with different concentrations of 5-FU along with 1 mM Mx for 24 hours. Next, the cells were irradiated with 2 Gy gamma radiation. After the treatments, we assessed for DNA damage, cytotoxicity, and viability by alkaline comet, clonogenic survival, and trypan blue dye exclusion assays

Results: Cytotoxicity and DNA damage increased with increasing 5-FU concentration. The 1 mM Mx concentration had no significant effect on cytotoxicity and DNA damage from 5-FU; however, it increased the cytotoxic and genotoxic effects of different concentrations of 5-FU when used in combination with 2 Gy gamma radiation

Conclusion: Mx combined with 5-FU enhanced the radiosensitivity of colon cancer cells

Comparison of metrezoate-labeled gallium transmission with different 50, 60 and 75% concentrations in malignant lymphatic cells

Radiation therapy is considered as one of the main methods in cancer treatment. In this method, determination of exact location and geometric properties of tumor is necessary for treatment planning that leads to increased accuracy and output of treatment and reduction of healthy tissue dose. For this purpose, the aim of the present study has been physiological transmission of Gallium as a pharmaceutical carrier which is bonded with an X-Ray opaque media for treatment of Lymphatic tumor via Smart Radiotherapy method [Photoelectron therapy]. Using radiography or CT scan has provided determination of geometric features of tumor

Diffusion tensor tractograghy can affect treatment strategy to remove brain occupying mass lesions

Radical resection of a pathological lesion along with the preservation of eloquent cerebral tissue is the principle goal of neurosurgery. Brain lesions are usually diagnosed by conventional magnetic resonance imaging [MRI], but this method is unable to describe the relationship between lesions and neighboring specific white matter [WM] tracts. Diffusion tensor tractograghy [DTT] is a new sophisticated imaging modality to reveal the neural fibers and their relationships with lesions. In the current study we assess that how diffusion tensor tractograghy can affect on treatment planning in patients afflicted by different types of brain lesions. In this prospective observational study, eight patients with brain mass lesion underwent conventional brain MRI pulse sequences and DTT imaging with 1.5 Tesla system using 64 independent diffusion encoding directions between December 2011 to January 2013.Acquired images were assessed by the neuroradiologist and neurosurgeon. Finally, the treatment strategies were compared using data before and after the tractograghy. The treatment strategy in six patients changed from radiotherapy into the craniotomy by using tractograghy data, in one patient changed from radio surgery to craniotomy and in one patient, neurosurgeon preferred to avoid operation. As we can infer from this study, based on the tractograghy results, the treatment technique may be changed, and the treatment plan could be devised with more accuracy and in case of surgery, may lead to less post-operative neurological deficits and better outcome results