Immunophenotyping of bone marrow biopsies in 26 patients with non-hodgkin's lymphoma, using anti-CD3, CD8, CD19 and CD20 monoclonal antibodies

Immunophenotyping with monoclonal antibodies [MoAbs] directed against lymphoid-associated antigens, immunohistochemical staining on paraffin-embedded BM biopsy material, and molecular studies of Ig genes/T-cell receptor genes or lymphoma-associated gene translocations should be used in the global approach to the patient with malignant lymphoma. 1. To determine the subtypes of non-Hodgkin lymphomas [B- or T-cell] in the bone marrow using anti-CD3, CD8 monoclonal antibodies for T-cell and anti-CD19 and CD20 for B-cells. 2. Correlation of the subtypes of NHL [B- or T-cell] with the morphology and pattern of bone marrow infiltration. A retrospective study, done in Al-Kadhymia teaching hospital during the period from 1/10/2010 to1/2/2011.The study consisted 26 adult patients, who were diagnosed as Non-Hodgkin lymphomas by undergoing a BM biopsy. Immunohistochemical staining of the paraffin-embedded sections of BM trephine biopsies was performed in all cases and used standard techniques with monoclonal anti-CD8, CD20 and dual immunofluorscence-labelled CD3, and CD19 antibodies and also all stained with Hematoxylin and Eosin [H and E] for morphologic assessment. The 26 cases of NHL comprised of 14 male [54%] and 12 female patients [46%]. The median age was [57.32] year ranged from 27-85 years. There were 23 cases of B-cell cases [88.5%] and 3 cases of T-cell lineage [11.5%] of all the cases. Among all the B-cell lymphomas, 15 cases showed interstitial infiltration in the bone marrow, while among the T-cell lymphoma two cases showed diffuse infiltration. 1. In these 26 cases NHL patients with marrow involvement, B cell phenotype comprised 88% of cases. 2. B-cell NHLs had predominance of interstitial infiltration in bone marrow biopsies in comparison with the T-cell lymphoma, in which diffuse infiltration was predominant

Physical intimate partner violence, prevalence and risk factors among rural women in Missan governorate, Iraq, 2009

Intimate Partner Violence [IPV], a most common form of violence against women, is a worldwide serious problem as a public health and human rights' concern. Health consequences of IPV are numerous and may be fatal It is a risk for unhealthy behaviors like smoking. This study aimed to determine the prevalence of lifetime physical IPV and potential risk factors among currently married women in rural Missan Governorate, south of Iraq 2009. A household population based cross sectional study was conducted in 2009 on a systematic random sample of 3345 households in rural Missan Governorate. One currently married woman aged 15-49 years was randomly selected from each sampled household. Physical IPV was defined as having any of the following experiences: was slapped or her arm twisted, pushed or shoved, kicked or dragged, punched, threatened or attacked with a weapon. Logistic regression models were used. Response rate was 96%; mean age 31.25 years [+/- SD 8.262]. Overall prevalence of lifetime physical IPV was [27.5%]. The most common form was pushing and shoving [23.2%]. Older women were significantly more prone to experience physical IPV. Those with 1 -6 live children had significantly higher risk of physical IPV. Experience of any husband's controlling behaviour increases the risk of physical IPV. Prevalence of physical IPV is high in rural Missan Governorate. A more in depth analytical study is recommended to determine other IPV risk factors and health consequences. Strengthening of the national programs aimed to empower women and community education regarding IPV is recommended