Treatment of paederus dermatitis with sambucus ebulus lotion

Paederus dermatitis is an irritant contact dermatitis due to accidental contact by a beetle belonging to the genus paederus. In this study, clinical efficacies of S. ebulus fruit extract solution in patients affected by paederus dermatitis were evaluated. A randomized double-blind, prospective, placebo-controlled clinical trial was performed in 62 patients with clinical symptoms and sings of dermatitis due to paederus beetles. The patients received either a topical solution of palemolin [a 5% S. ebulus fruit extract in ethanol 70%] or ethanol 70% topical solution thrice a day. Topical hydrocortisone ointment was prescribed for all patients. Palemolin was statistically more effective in controlling of burning, pain, inflammation, drying the wound, infections and acceleration of healing than control group [p

Effects of total extract of Dracocephalum moldavica on ischemia/reperfusion induced arrhythmias and infarct size in the isolated rat heart

Dracocephalum moldavica [D. moldavica] have been traditionally used as a cardiotonic agent in the folk medicine of some regions of Iran. In the present study, effects of total extract of D. moldavica on ischemia/reperfusion induced arrhythmias and infarct size investigated in isolated rat heart. The isolated hearts were mounted on a Langendorff apparatus then perfused during 30 min regional ischemia and 120 min reperfusion, either by a modified Krebs-Henseleit solution as the control group or by enriched Krebs solution with total extract of D. moldavica [25-200 micro g/ml] as the treatment groups. The ECGs recorded and analyzed to determine cardiac arrhythmias. At the end of the reperfusion, the hearts stained by Evans blue solution then incubated by triphenyltetrazolium chloride. The volume of infarcted tissue and percentage of infarct size determined by computerized planimetry. The results demonstrated that total extract of D. moldavica caused a significant reduction in the number of ventricular tachycardia [VT], total ventricular ectopic beats [VEBs] and VT duration in ischemic and reperfusion periods. The incidence of ischemic VT reduced from 93% in the control group to 0, 50 and 50% in the treatment groups. The infarct size was 37 +/- 1% in the control group, however, perfusion of the extract [25, 50, 200 micro g/ml] reduced it to 13 +/- 2, 8 +/- 1 and 18 +/- 2%, respectively [P < 0.001]. In addition, the extract remarkably lowered volume of infarcted tissue compared to the control group [P < 0.05]. Our findings showed cardioprotective effects of total extract of D. moldavica against ischemia/reperfusion injuries in the isolated rat heart

investigation on methylation methods of hesperidin

Since hesperidin is a poor water soluble compound, in pharmaceutical formulations its methylated derivatives [hesperidin methyl chalcone, HMC] are used. The aim of this study was to establish an efficient methylation method for preparation of hesperidin methyl derivatives. For this purpose hesperidin was isolated from tangerine peel, purified and its methyl derivatives were prepared using three different techniques, i.e. diazomethane, methyl iodide-sodium hydride and dimethylsulfate. The efficiency of the methods was evaluated in terms of the percentage of unchanged and intact hesperidin in the final methylated products the and amount of unchanged hesperidin was an indication of the better efficiency of the method. A reversed phase HPLC method was also developed for determination and quantification of hesperidin in the final methylated products.The method involved the use of a Shim pack CLC-ODS column, a mixture of methanol-phosphate buffer [37:63, v/v] of pH = 2.6 as a mobile phase in an isocratic mode at a flow rate of 1 ml/min and UV detection at 280 nm. The results showed that methylation with methyl iodide-sodium hydride have the highest efficiency among different methylation methods