Characterization of Mesenchymal Stem Cells Derived from Rat Bone Marrow and Adipose Tissue: A Comparative Study

BACKGROUND AND OBJECTIVES: Stem cell technology offers a new hope for many chronic disorders patients. The types of stem cells are different with many differences existing between each type. Mesenchymal stem cells (MSCs) represent one type of adult stem cells that can be easily isolated, then re-transplanted to the patients. This offers potential for their future application in treating many disorders without fear of rejection possibility. MSCs can be isolated from different sources e.g. bone marrow (BMSCs) and adipose tissue (ADSCs). In the present study we compared BMSCs and ADSCs isolated from Sprague-Dawley rats. METHODS AND RESULTS: For this comparison, immunophenotyping, the analysis of growth rates, proliferation by colony forming unit-fibroblast assay, population doubling time, and trilineage differentiation assays were performed for both BMSCs and ADSCs. The findings revealed that despite no difference in immunphenotypic character between BMSC and ADSC, a better proliferative capacity was observed for ADSCs which would advocate their better use in regenerative applications. On the other hand, BMSCs showed more potential for osteogenic and chondrogenic differentiation. CONCLUSIONS: Our study showed that, despite many similarities between both types of cells, there are differences existing which can offer assistance on choosing type of cell to be used in specific diseases. Although ADSCs seem more promising for regenerative application generally, BMSCs may represent a better choice for treating bone disorders.

Assessment of renal function with rheumatic heart disease

45 Children suffering from rheumatic fever and its sequele were chosen for the study of renal function and the results were compared with samples taken from 15 healthy children [controls]. Full history, clinical examination, chest x-ray, ECG, routine investigations including complete blood picture, ESR, ASOT, and specific investigations for renal function namely blood urea nitrogen, serum creatinine level, and beta2 microglobulin level were done for every subject in the study. The serum creatinine level showed no statistical significance - while beta2 micro globulin [beta2 MG] showed statistical significant values when we compared the patients with the control group also the blood urea nitrogen showed statistically significant results. Although BUN, and beta2 MG are statistically significant, yet the results do not indicate either renal failure or glomerular insufficiency

Biochemical study on the effect of diazepam on serotonin, dopamine, norepinephrine and cholinesterase enzyme contents of rat brain

The levels of serotonin, dopamine, norepinephrine and cholinesterase in the brain of adult male albino rats injected intraperitoneally with Diazepam [10 mg/kg body weight, valium, Rochi] were studied after one and twelve hours post injectin. Diazepam exerted no significant change in brain cholinestrase activity, while a highly significant increase in brain serotonin [5 HT], dopamine and norepinephrine activities in male albino rats one hour after injection, was recorded, 36.9%, 30.9%, and 43.3% respectively, P < 0.001 such increased levels have been restored to normal levels twelve hours after administration

Changes of ascorbic acid content in serum and suprarenal gland of albino rats after injection of snake venoms

LD50 doses of B. arietans, B. gabonica, D. polylepis, N. haje, N. nigricollis. O. cerastes and C. viper snake venoms caused a significant depletion of ascorbic acid in the suprarenal gland from 28.47 percent to 70.87 percent while the same doses of the different venoms showed no significant changes in the ascorbic acid content in rat serum