RESUMO
Aim: This study aimed to investigate the association of IFN- gamma +874 [T/A] polymorphism with susceptibility to chronic HBV infection in the Syrian population
Background: Accumulating evidence indicate that the inadequate immune responses are responsible for HBV persistency. Therefore, polymorphisms in genes encoding the cytokines, which are responsible for regulation of the immune response, can affect the course and outcome of the infection. The IFN-gamma +874 T/A polymorphism affects the expression of IFN-gamma, which has been shown to be crucial to HBV clearance
Methods: In this case-control study, 140 samples were collected [70 healthy individuals, 70 chronic HBV patients], and genomic DNA was isolated. Sequencing and ARMS-PCR were performed to genotype the IFN-gamma +874 T/A polymorphism
Results: Results of this study showed an association between IFN- gamma +874 T/A polymorphism and the susceptibility to chronic HBV infection [P < 0.05]. In addition, results showed that the AA genotype increased the risk of chronicity [OR = 3.05, 95% CI = 1.35 - 6.89], whereas the AT and TT genotypes reduced the risk of chronicity [OR = 0.33, 95% CI = 0.150 - 0.753]
Conclusion: Results of this study conclude that the IFN- gamma +874 T/A polymorphism may be associated with the chronic HBV infection, according to the genetic model AA vs. ATandTT
RESUMO
Hepatitis B is an infectious disease to which dentists are susceptible. The aim of this study was to determine the level of hepatitis B surface antibodies titer and immunity degree in some of vaccinated students who receive the regular and obligate vaccination in faculty of dentistry-Damascus university and study its relationship with some variables. 91 blood samples were examined by Microparticle enzyme immunoassay [MEIA] to determine the quantity of the HBs antibodies, these obtained levels of antibodies were considered according to their titers as nonreactive, acceptable, good and very good. This study showed that 7.7% of vaccinated students did not respond to vaccination and the levels of antibodies were higher in females [P=0.037], and higher in nonsmoking students [P=0.024] with statistically significant correlation, whereas alcohol consumption, body mass index [BMI] and the interval between the last vaccination dose and the commencement of this study had no statistically significant correlation. Our results concluded that smoking is a risk factor on the immune response towards vaccination. This study highlight the need for doing Anti-HBs test after receive the vaccine and we recommend that Postvaccination test is to be included in routine immunization services of school of dentistry, Damascus university
RESUMO
Blood transfusion is considered one of the main risk factors, which causes infection of blood-born viruses, mainly HCV and HBV. The study included 75 children [ages between 1-14 years], admitted to Pediatric Hospital in Damascus. Those patients had different hematological diseases like Thalassemia and Sickle Cell Anemia. Serum ALT, AST, and total bilirubin were tested, and the results were compared to the normal range values of those parameters, which were determined during the study. Viral markers [anti-HCV, HRsAg, anti HBc] were tested using enzyme linked immuno sorbent assays. The results showed that mean values of biochemical parameters in those patients were higher than mean values of normal range of those parameters. Ten out of seventy-five patients [13.3%] were positive for anti-HCV, 4 / 75 [5.3%] were positive for HBs Ag, and 2/75 [2.6%] were positive for anti-HBc, only one case [1.3%] was positive for both anti-I and HBsAg. There was a significant correlation between anti-HCV positivity, and number of blood transfusions [P<0.05] Whereas, there was no such correlation between age, sex of patients and anti-HCV positivity. Results showed that hepatitis viruses infection is common among hematological diseases patients due to repeated blood transfusion. Diagnostic assays adopted by Syrian blood banks will decrease the prevalence of these viruses among those patients