Biochemical study on normal and leukemic Egyptian children

Leukemia is the most common malignancy of childhood [33%] where Acute Lymphoblastic Leukemia [ALL] is the most common type in children. is to study the effect of ALL on complete blood count [CBC] and on some important biochemical parameters as total protein, albomin, globulin, uric acid, lactate dehydrogenase [LDH], erythrocyte superoxide dismutase [eryth. SOD] and erythrocyte glucose-6 -phosphate dehydrogenase[eryth. G-6-PD]. Also to measure total DNA and RNA content in all the samples. Moreover to carry out a comparative study of these parameters before and after treatment in contrast to control group. This study included forty newly diagnosed children with [ALL] in National Cancer Institute [NCI], Cairo University. Blood samples on admission [Gr. IIA] and after 4 weeks of chemotherapeutic treatment [Gr. IIB] were analyzed. Four cases died 2 weeks from diagnosis, one case discontinued, and one case failed to remission induction. CBC revealed a significant remarkable decrease in the hemoglobin content, RBCs and platelet count in [Gr. IIA] and these parameters were improved in [Gr. IIB]. A significantly marked elevation in WBCs and blast cells were recorded in [Gr. IIA]. On remission they returned to their normal level in [Gr. IIB]. Total DNA and RNA contents revealed a significant remarkable elevation in [Gr. IIA], while after 4 weeks of treatment they returned to normal level [Gr. IIB]. G-6-PDH showed significant increase in [Gr. IIA] while SOD activity showed high significant decline in [Gr. IIA]. After treatment both G- 6-PD and SOD showed significant improvement [Gr. IIB]. One month of chemotherapeutic treatment is not enough to achieve complete hematological remission for Egyptian [ALL] children in contrast to European reports. Biochemical parameters returned to their normal level, yet G-6-PD and SOD activities were improved largely. Also measurement of G-6-PD and SOD activities and DNA and RNA contents are useful markers to follow up ALL children after chemotherapeutic treatment

study on the thyroid function in male infertility

This study was carried out on 20 normal control, twenty oligospermic subjects [sperm count < 20x 10[6]]; and ten azospermic individuals. Serum triiodothyronine and tetraiodothyronine; thyroxine [T3 and T4] were estimated as parameters for the thyroid function.Motility and sperm count were carried out on semen specimens. Serum total proteins, albumin, the albumin/globulin ratio [A/G] ratio and calcium were also determined. This study pointed to a relationship between infertility and hyperthyrodisim in the oligospermic groups. Results are tabled and discussed