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1.
Chinese Journal of Stomatology ; (12): 343-347, 2013.
Artigo em Chinês | WPRIM | ID: wpr-293592

RESUMO

<p><b>OBJECTIVE</b>To investigate the effects of mineral trioxide aggregate(MTA) on the proliferation and differentiation of rat dental papilla cells(RDPC).</p><p><b>METHODS</b>RDPC were cultured by tissue block method and identified.RDPC of the third passage were cultured with material extract fluid containing different mass concentrations of MTA (0.002, 0.02,0.2, 2 and 20 g/L) for 3 d, those cultured with routine culture fluid served as control group. The proliferation-related parameters were measured by methyl thiazolyl tetrazolium(MTT) assay. RDPC were cultured with material extract fluids containing 0.002 g/L MTA, those cultured with routine culture fluid served as control group, the activity of alkaline phosphatase(ALP) at 1, 3,5, 7 d and the level of collagen I at 1, 3,5 d were detected.</p><p><b>RESULTS</b>MTT results showed that the A value of RDPC of group 20 g/L (0.092 ± 0.011) was less than that of the control group (0.249 ± 0.006) at 3 d(P < 0.01), the A value of RDPC of group 0.02 g/L (0.267 ± 0.005) and 0.002 g/L (0.276 ± 0.006) were more than that of the control group (0.249 ± 0.006) at 3 d(P < 0.01). ALP detection proved that ALP activity of MTA at 3 d (0.217 ± 0.008), 5 d (0.253 ± 0.005) , 7 d (0.279 ± 0.004) were more than that of the control group at 3 d (0.166 ± 0.006) ,5 d (0.221 ± 0.006), 7 d (0.242 ± 0.004) (P < 0.01). Collagen I detection showed that the level of collagen I of MTA at 3 d[(78.46 ± 2.72) µg/L], 5 d[(90.73 ± 3.08) µg/L] were more than that of the control group at 3 d[ (66.75 ± 3.08) µg/L], [5 d (74.27 ± 3.50) µg/L] (P < 0.01).</p><p><b>CONCLUSIONS</b>MTA of high concentrations can significantly inhibit cell growth, and of low concentrations can promote cells proliferation and differentiation.</p>


Assuntos
Animais , Feminino , Masculino , Ratos , Fosfatase Alcalina , Metabolismo , Compostos de Alumínio , Farmacologia , Compostos de Cálcio , Farmacologia , Diferenciação Celular , Proliferação de Células , Células Cultivadas , Colágeno Tipo I , Metabolismo , Papila Dentária , Biologia Celular , Metabolismo , Relação Dose-Resposta a Droga , Combinação de Medicamentos , Óxidos , Farmacologia , Ratos Sprague-Dawley , Silicatos , Farmacologia
2.
China Journal of Chinese Materia Medica ; (24): 853-856, 2003.
Artigo em Chinês | WPRIM | ID: wpr-282226

RESUMO

<p><b>OBJECTIVE</b>To study the effect of Daidzein on Antiarrhythmia.</p><p><b>METHOD</b>The conventional antiarrhythmia methods were used.</p><p><b>RESULT</b>Daidzein was remarkedly effective in preventing ventricular fibrillation induced by chloroform in mice and arrhythmia induced by aconitine in rats. The arrhythmia induced by adrenalin in rabbits was antagonized by Daidzein and it could obviously inhibit the action potential amplitude of isolated sciatic nerves in toads. And it could also prevent ventricular fibrillation induced by calcium chloride in rats, and obviously reduce the death rate of rats. Its anti-arrhythmic effect was dose-dependent.</p><p><b>CONCLUSION</b>Daidzein has obvious protective effect on drug-induced arrhythmia, which may be related to its inhibition of Na+ or Ca2+ influx and its blocking beta-adrenergic receptor.</p>


Assuntos
Animais , Feminino , Masculino , Camundongos , Coelhos , Ratos , Aconitina , Antiarrítmicos , Usos Terapêuticos , Arritmias Cardíacas , Bufo bufo , Cloreto de Cálcio , Clorofórmio , Epinefrina , Técnicas In Vitro , Isoflavonas , Usos Terapêuticos , Fitoterapia , Plantas Medicinais , Química , Pueraria , Química , Ratos Wistar , Fibrilação Ventricular
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