RESUMO
OBJECTIVE: To compare the HPLC fingerprints Aconitum soongaricum Stapf. with Aconitum carmichaelii Debx., Aconitum kusnezoffii Reichb., and another common radix aconite plant in Xinjiang, Aconitum leucostomum Worosch., and analyze the degree similarity between different fingerprints. METHODS: The HPLC fingerprint Aconitum soongaricum Stapf was established, and similarity analysis, cluster analysis and principal component analysis were carried out for the common patterns, Aconitum soongaricum Stapf and Aconitum carmichaelii Debx., Aconitum kusnezoffii Reichb., and Aconitum leucostomum Worosch. via the ChemPattem chemical fingerprint analysis system software solutions. RESULTS: The analysis showed that the similarities the mutual model reference fingerprints were higher between Aconitum soongaricum Stapf and Aconitum kusnezoffii Reichb. and between Aconitum leucostomum Worosch. and Aconitum carmichaelii Debx. CONCLUSION: It is preliminarily determined that Aconitum soongaricum Stapf in Xinjiang had higher similarity with the legal standard Aconitum kusnezoffii Reichb., which deserves further research and development.
RESUMO
OBJECTIVE: To establish the HPTLC fingerprint of Aconitum plants in Xinjiang and to carry out similarity comparison, cluster analysis and principal component analysis. METHODS: Using high performance thin layer silica gel G plate, n-hexane-ethyl acetate-methanol (6.4:3.6:1) as developer, diluted bismuth potassium iodide solution as colour-developing agent, of the HPTLC fingerprints were obtained. The common pattern was explored by chemical fingerprint analysis system software. Similarity analysis, cluster analysis and principal component analysis were carried out. RESULTS: The conditions for thin layer chromatography were optimized, the spots were clear, and the separation was good; similarity analysis showed that Xinjiang Aconitum had some differences in chemical composition between different plant species, which can be broadly divided into three categories. Aconitum leucostomum Wo-rosch. and Aconitum apetalum (Huth) B. Fedtsch. had high similarity with Aconitum carmichaelii Debx. and Aconitum kusnezojfii Reichb. CONCLUSION: The established thin-layer chromatographic fingerprints have laid the methodological foundation for the study of the fingerprint of Xinjiang Aconitum plants. Aconitum leucostomum Worosch, which is a widely distributed and abundantly reserved Aconitum species in Xinjiang, has potential medicinal value and similarity with Aconitum carmichaelii Debx. and Aconitum kus-nezoffii Reichb. Which have national quality standard, and is worthy of further studies.