Clinical features of acquired immunodeficiency syndrome patients complicated with peripulmonary occupational lesions / 中华传染病杂志

Objective:To analyze the clinical features of acquired immunodeficiency syndrome (AIDS) patients complicated with peripulmonary occupational lesions.Methods:Fifty-five AIDS patients with peripulmonary occupational lesions treated in Guangzhou Eighth People′s Hospital from January 2012 to January 2019 were included, and the clinical data of patients were retrospectively analyzed. According to the results of lung biopsy, the patients were divided into Mycobacterium infection group, fungal infection group and tumor group. The clinical characteristics, the proportion of different CD4 + T lymphocyte counts and chest computed tomography (CT) features of the three groups were compared. Chi square test was used for comparison among the three groups, and Bonferroni method was used to correct the test level for pairwise comparison. The significance level was 0.016 7 because of three pairwise comparisons. Results:Among 55 AIDS patients complicated with peripulmonary occupational lesions, pulmonary biopsy showed 14 cases with Mycobacterium infection, 12 cases with fungal infection and 15 cases with tumor lesions. Mixed diseases were found in 11 patients, including seven cases with Mycobacterium and fungus coinfection, four with tumor complicated with fungus and (or) Mycobacterium. Three with chronic interstitial pneumonia. The main clinical manifestations of 55 patients were fever, expectoration, fatigue, weight loss and superficial lymph node enlargement. There were no significant differences in symptoms/signs, white blood cell counts, hemoglobin levels, alanine transaminase and creatinine among Mycobacterium infection group, fungal infection group and tumor group (all P>0.05). There was significant difference in anti-retroviral therapy (ART) acceptance among the three groups ( χ2=15.165, P<0.01). However, the results of pairwise comparison between groups showed that there was significant difference between fungal infection group and tumor group ( χ2=7.514, P<0.016 7), while there was no significant difference between Mycobacterium infection group and tumor group, Mycobacterium infection group and fungal infection group ( χ2=0.255 and 5.306, respectively, both P>0.016 7). There were significant differences in clinical outcomes among the three groups ( χ2=15.119, P<0.01), and the pairwise comparison between the Mycobacterium infection group and the tumor group, and the fungal infection group and the tumor group showed significant differences ( χ2 =10.311 and 9.095, respectively, both P<0.016 7). The cases with CD4 + T lymphocyte count ≤50/μL, 51-<200/μL and ≥200/μL in Mycobacterium infection group were three cases, one case and 10 cases, respectively; those in fungal infection group were 10 cases, two cases and 0 case, respectively, and those in tumor group were one case, two cases and 12 cases, respectively. The difference was statistically significant ( χ2=21.284, P<0.01). Chest CT showed that there was significant difference in the types of space occupying lesions among the three groups ( χ2=13.308, P=0.003), and pairwise comparison between the two groups showed that there was significant difference between the Mycobacterium infection group and the tumor group ( χ2=11.312, P<0.016 7), while there were no significant differences between the Mycobacterium infection group and fungal infection group ( χ2=0.931, P>0.016 7), and the fungal infection group and the tumor group ( χ2=7.053, P>0.016 7). There was significant difference among the three groups in calcification focus ( χ2=8.524, P=0.004), while there was no difference between the Mycobacterium infection group and fungal infection+ tumor group ( χ2=10.982, P<0.016 7). Conclusions:Mycobacterium infection, fungal infection and tumor are the main types of peripulmonary occupational lesions in AIDS patients. The differential diagnosis could be made by combining with chest CT features, ART acceptance and CD4 + T lymphocyte level.

Analysis on disease spectrum among hospitalized children in Chongqing City during 2015 / 重庆医学

Objective To study the characteristics of the diseases spectrum in hospitalized children.Methods The items of main diagnosis,gender,age,etc.in the inpatients medical records home page data aged 0? 12 years old in second-level and above hospitals of Chongqing City during 2015 were performed the disease spectrum sequence and constituent ratio analysis for deeply digging the relation between the disease change trend with the age and sex.Results The top 5 main system diseases affecting children's health were respiratory system diseases (male 43.84％,female 45.74％),some conditions originated from prenatal period (male 12.01 ％,female 13.87 ％),infectious diseases and parasites diseases (male 9.48 ％,female 9.88 ％),digestive system diseases (male 9.09 ％,female 6.95 ％),injury,poisoning and some other consequences of external causes(male 6.86 ％,female 5.27 ％).Respiratory system diseases always ranked the first place,the sexual difference among various system disease was unobvious,the sequence of injury,poisoning and some other consequence of external causes was increased with the age increasing;in the ranking of respiratory system diseases,pneumonia(38.73 ％),acute bronchitis(17.29 ％) and acute tonsillitis(13.83％) were the top 3 diseases affecting children's health.Conclusion Respiratory system diseases are the concerned key point for children health promotion.The health and epidemic prevention departments should provide effective methods and measures for preventing respiratory system diseases in children.Furthermore,the targeted prevention and treatment measures should be proposed aiming at the difference of disease sequence in different age groups.

Prognostic factors for patients with acquired immunodeficiency syndrome-related lymphoma / 中华传染病杂志

Objective To explore the risk factors for the prognosis of acquired immunodeficiency syndrome (AIDS)-related lymphoma (ARL).Methods A total of 32 patients with ARL were enrolled in this study from Guangzhou Eighth People's Hospital during August 2011 and July 2014,who were followed up for 2 to 48 months (median 9 months).The risk factors for the prognosis of ARL were analyzed by Logistic regression analysis.Results A total of 32 patients were included,among whom 27 were males and the rest were females,with a mean age of (42.8± 13.5) years (ranging from 4 to 70 years).Regarding the clinical staging at the initial diagnosis,6 cases (18.8 ％) were classified as stage Ⅰ,4 cases (12.5％) stage Ⅱ,5 cases (15.6％) stage Ⅲ,and 17 cases (53.1％) stage Ⅳ.As for international prognostic index (IPI) score,4 cases (12.5％) were in the low risk group (IPI 0-1),5 cases (15.6％) in the low-middle group (IPI 2),8 cases (25.0％) in the middle-high risk group (IPI 3),and 15 cases (46.9 ％) in the high risk group (IPI 4-5).According to the pathological diagnosis,26 cases (81.2％) were B cell non-Hodgkin lymphoma (NHL),4 cases (12.5％) were T cell NHL,and 2 cases (6.2％) were Hodgkin lymphoma (HL) nodular sclerosis.In total,23 cases (71.9％) had received highly active antiretroviral therapy (HAART) before the first hospital visit or within one month after the diagnosis of lymphoma.Sixteen (50.0％) cases received standard chemotherapy regimen formulated by the oncology specialists.A total of 17 patients died,with the mortality rate of 53.1％.By univariate analysis,the prognostic factors included age (OR=0.915,P=0.012),high lactate dehydrogenase (OR=1.006,P=0.021) value at baseline,receiving HAART (OR =12.444,P=0.011),and standard chemotherapy regimen (OR=13.000,P=0.001).By multivariate Logistic regression,receiving standard chemotherapy regimen (OR=0.035,P=0.022) was the only prognostic factor of ARL.Conclusions The mortality of ARL is high and the most common pathological type was NHL.The prognosis could be improved by standard chemotherapy regimen on the basis of HAART.

The Distributed Naive Bayesian Intelligent Diagnosis System Based on Hadoop / 医学信息学杂志

The paper introduces the research idea, design and realization of the distributed Naive Bayesian intelligent diagnosis sys-tem based on Hadoop, makes optimization and improvement according to its application in Traditional Chinese Medicine ( TCM) Hospital of Guangdong Province, including algorithm design improvement and enhancement of accuracy, extensibility and security of the system.

Study on hierarchical and mutually supplementary appropriate technology in health screening based on diagnosis and treatment of disease in Chongqing / 重庆医学

Objective To screen the hierarchical,mutually supplementary Appropriate Technology in Health suitable for the promotion and application in the county and township two-level medical institutions in Chongqing for gradually establishing and per-fecting the grading and classification of Appropriate Technology in Health database,and expanding Appropriate Technology in Health reserves.Methods Using Lv Yalan[1] assessment index system of appropriate health technology,then the experts′scoring method in the field was adopted for evaluating and screening the Chongqing Municipal Science and Technology Proj ectbased on stratification of diagnosis and treatment of disease complementary appropriate technology research,evaluation and integration dem-onstrationAppropriate Technology in Health group[2].Results The comprehensive scores of various techniques were high,in which the lowest score was 94.61 points,including:the drug seretide inhalation technique 98.24 points,the drug salbutamol inhala-tion technique 98.56 points,inhaled medications to control asthma technique 97.08 points,the drug spiriva(tiotropium)inhalation technique 97.97 points,6-min walk test 96.31 points,pulmonary ventilation function inspection technique 95.61,summary pulmo-nary function testing technique 94.59,children bronchial get up early screening technique 94.59,child bronchial asthma detection technique-C-AT(children′s asthma control test)evaluation form 96.94.Conclusion According to the inclusion criteria for Appro-priate Technology in Health and the experts′scores,9 items of Appropriate Technology in Health included by preliminary screening are selected for putting in storage,combined with the health resources and the prevalence rate in the demonstration counties and townships,these 9 items of technology may be promoted and applied.

PEGylation and immunological characterization of rBla g 2 allergen / 中华微生物学和免疫学杂志

Objective To research the effect of PEGylation on rBla g 2 from Blattella germanica.Methods rBla g 2 allergen expressed in E.coli was purified by Ni+affinity chromatographY,then was PEGylated by mPEG2-NHS(Mr,10×103).The PEG-rBla g 2 was purified by CM-Sepharose,a cation exchange chromatography. SDS-PAGE,Western blot and ELISA were used to characterize its biologicat actovoty.Results The relative molecular mass of the purified rBla g 2 was about 39×1023.PEG-rBla g 2 was analyzed by SDS-PAGE.Five bands were visualized by staining with Coomassie Brilliant Blue R-250,while seven bands by staining with I2-KI.Cation exchange chromatography could separate rBla g 2 and PEG-rBla g 2.The 100×103(Mr)and 130×103(Mr)of PEG-rBla g 2 could combined with the special IgE from sera of one cockroach-allergic patient by Western blot.The immunological activities of PEG-rBla g 2 in vitro decreased remarkablv bv ELISA,which was only 42%of rBla g 2.Conclusion PEGylated allergen can retain the ability of combining with special IgE from sera,while its immunological activities reduce enormously,which establishes the basic work of researching recombinant low-sensitive allergens.

Determination of Fluvastatin Sodium Extended-release Tablets by HPLC / 中国药房

OBJECTIVE:To establish a HPLC method for determining the content of fluvastatin sodium in fluvastatin sodium extended-release tablets. METHODS:An Agilent SB-C18 column was used and the mobile phase was CH3CN-0.1% phosphate acid, 50∶50. Flow rate was 1mL?min-1, wavelength was 234nm and the sample size was 20?L. RESULTS:The fluvastatin sodium concentration had good liner relationship from 4.84～96.8?g?mL-1(r=0.999 9), and the average recovery was 100.1%,RSD=0.9%. CONCLUSION:This method was simple, fast, accurate, and precise, could be used to determine content in fluvastatin sodium extended-release tablets.