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Objective:To observe the intervention mechanism of phlegm-stasis co-treatment for the JNK signaling pathway in the myocardium of diabetes rats.Methods:Totally 50 male SD rats of SPF grade were selected. Diabetes model was established by single intraperitoneal injection of 55 mg/kg streptozotocin (STZ) solution. After continued feeding for 3 weeks, the rats were divided into normal group, model group, alachloramine group, blood stasis removing group, phlegm removing group and phlegm-blood stasis co-treatment group according to random number table method, with 6 rats in each group. Xiaoxianxiong Decoction (4.05 g/kg), Xuefu Zhuyu Decoction (7.02 g/kg), Didang Xianxiong Decoction (8.10 g/kg) were administered to the stomach respectively in the phlegm removing group, the blood stasis removing group and the phlegm-blood stasis co-treatment group. Alachloramine (3 mg/kg) was administered to the stomach by gavage in the alachloramine group. After 8 weeks, HE staining was used to observe the morphological changes of myocardial tissue in diabetic rats. Masson staining was used to observe the deposition of collagen fibers in the myocardial interstitium in rats. The expression of JNK1 protein was determined by immunohistochemistry. JNK1 mRNA, IRS1 mRNA and NLRP3 expression levels were detected by Real-time PCR. Western blot was used to detect the protein expressions of IRS-1, p-Akt and NLRP3.Results:The myocardial cells in the model group were disorganized, with hypertrophy, blurred texture, inflammatory infiltration of interstitium, increased collagen fibers, and focal necrosis. All treatment groups could improve fibrosis, inflammatory infiltration and reduce myocardial collagen deposition in different degrees. Compared with the model group, the mRNA and protein expressions of JNK1 and NLRP3 bodies decreased ( P<0.01), the IRS-1 mRNA and protein increased ( P<0.01), and p-Akt protein expression increased ( P<0.01). Conclusions:The phlegm and stasis co-treatment can effectively improve the cardiomyopathy of diabetes rats, and the effect is better than the phlegm-resolving method or the stasis resolving method alone. The mechanism may be related to the inhibition of JNK signaling pathway activation, reduce the expressions of JNK1 and NLRP3, and increase the IRS-1 and Akt.
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Objective:To investigate the effect of metoprolol tartrate combined with atorvastatin calcium on lipid metabolism, vascular endothelial function and inflammatory factors in patients with essential hypertension.Methods:From January 2015 to December 2018, 80 patients with essential hypertension admitted to the People's Hospital of Dongyang were divided into observation group (40 cases) and control group (40 cases) according to the random digital table method.On the basis of routine treatment, the control group was treated with atorvastatin calcium, while the observation group was treated with atorvastatin calcium and metoprolol.The course of treatment in both two groups was 6 months.The changes of systolic and diastolic blood pressure, lipid metabolism, endothelial function and inflammatory factors were compared before and after treatment.Results:The total effective rate of the observation group (92.50%, 37/40) was higher than that of the control group (70.00%, 28/40) (χ 2=6.646, P<0.05). The systolic blood pressure [(123.15±7.89) mmHg] and diastolic blood pressure [(75.52±3.89)mmHg] in the observation group were lower than those in the control group [(134.29±10.31)mmHg and (82.31±4.04)mmHg] ( t=5.427, 7.657, all P<0.05). The level of serum NO [(69.74±5.46)nmol/L] in the observation group was higher than that in the control group [(57.87±3.67)nmol/L], while ET-1 [(56.48±5.49)mg/L] in the observation group was lower than that in the control group [(73.24±7.18)mg/L], the differences were statistically significant( t=11.411, 11.728, all P<0.05). The serum levels of CRP [(1.18±0.23)mg/L], TNF-α [(0.63±0.18)μg/L] and IL-6 [(84.35±17.28)ng/L] in the observation group were lower than those in the control group [(1.64±0.45)mg/L, (1.23±0.26)μg/L and (128.43±19.86)ng/L] ( t=5.757, 12.000, 10.590, all P<0.05). Conclusion:Metoprolol tartrate combined with atorvastatin calcium is effective in the treatment of essential hypertension, and can improve lipid metabolism, vascular endothelial function, and reduce inflammation.
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Objective To investigate characteristics of intestinal flora in elderly patients with coronary heart disease (CHD) versus CHD plus complicated heart failure (HF).Methods The 80 CHD patients admitted into our hospital from April 2017 to April 2018 were consecutively enrolled in this prospective study.They were divided into the C HD group (without HF,n=40) and the HF group (CHD plus HF,n=40),and 40 healthy elderly people taking health examination during the same period were considered as the control group.The 0.5 g stool specimen from each observer was collected.The specific DNA fragments of 16S rDNA/18S rDNA/ITS/ function genes were amplified,the Polymerase Chain reaction(PCR)amplified products were sequenced by Illumina Genome Analyzer IIx.And the sequencing results were followed by Read splicing,OTU clustering,alpha diversity analysis and species diversity analysis.Finally,the sample species information was obtained.Results The mean abundances of gut microflora in CHD group and in HF group were lower than in control group (P＜0.05).The abundance of phylum bacteroidetes was higher in the control group (66.41± 3.25)％ than in the CHD group (45.21±9.07)％ and in HF groups (27.29±13.27)％(P＜0.05).And the abundance of phylum firmicutes was lower in the control group than in the CHD and HF groups (P＜0.05).LEfSe analysis showed that the flora with high abundances in the healthy elderly group were mainly Bacteroides,Bacteroidia and Bacteroidales.Ruminococcus and Clostridium occurred mainly in the CHD group,and Firmicutes and Enterobacte occurred mainly in CHD plus heart failure group.Conclusions The intestinal flora disturbance occurs in elderly patients with coronary heart disease or CHD with heart failure.
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Objective@#To investigate characteristics of intestinal flora in elderly patients with coronary heart disease (CHD) versus CHD plus complicated heart failure (HF).@*Methods@#The 80 CHD patients admitted into our hospital from April 2017 to April 2018 were consecutively enrolled in this prospective study.They were divided into the CHD group (without HF, n=40) and the HF group (CHD plus HF, n=40), and 40 healthy elderly people taking health examination during the same period were considered as the control group.The 0.5 g stool specimen from each observer was collected.The specific DNA fragments of 16S rDNA/18S rDNA/ITS/ function genes were amplified, the Polymerase Chain reaction(PCR)amplified products were sequenced by Illumina Genome Analyzer IIx.And the sequencing results were followed by Read splicing, OTU clustering, alpha diversity analysis and species diversity analysis.Finally, the sample species information was obtained.@*Results@#The mean abundances of gut microflora in CHD group and in HF group were lower than in control group (P<0.05). The abundance of phylum bacteroidetes was higher in the control group (66.41±3.25)% than in the CHD group (45.21±9.07)% and in HF groups (27.29±13.27)%(P<0.05). And the abundance of phylum firmicutes was lower in the control group than in the CHD and HF groups (P<0.05). LEfSe analysis showed that the flora with high abundances in the healthy elderly group were mainly Bacteroides, Bacteroidia and Bacteroidales.Ruminococcus and Clostridium occurred mainly in the CHD group, and Firmicutes and Enterobacte occurred mainly in CHD plus heart failure group.@*Conclusions@#The intestinal flora disturbance occurs in elderly patients with coronary heart disease or CHD with heart failure.