RESUMO
Objective: Adiponectin (APN) is an endogenous cytokine that mediates the development and progression of various tumors through its receptors (AdipoRs). The present study aimed to detect the expression and distribution of APN and its receptors (AdipoR1 and AdipoR2) in tongue squamous cell carcinoma (TSCC). Moreover, we explored whether the locoregional expression of APN was reg-ulated by HIF-1α in the hypoxic microenvironment. Methods: The expression and distribution of APN and its receptors in TSCC tissues were analyzed by immunohistochemical. Lentiviral expression vector for HIF-1α shRNA was constructed and stably transfected in TSCC cells to knock down HIF-1α expression. The mRNA and protein expression levels of APN and its receptors were detected using RT-PCR and Western blot, respectively, after hypoxic treatment. Results: The locoregional expression of APN and AdipoR1, but not AdipoR2, was upregulated at the early stages of T1, T2, and/or N0 stage, respectively, in tumor tissues compared to that in control paracancer-ous tissues (P<0.05 or P<0.01). The expression of APN and AdipoR1, but not AdipoR2, in TSCC cells was up-regulated on hypoxic treat-ment. Moreover, the expression of APN and AdipoR1 was down-regulated after shRNA knockdown of HIF-1α under hypoxia. Conclu-sions: The APN-AdipoR1 signaling pathway was activated and regulated by HIF-1α in the hypoxic environment of TSCC tissues.
RESUMO
Objective To investigate the role of curcumin in macraphage polarization induced by breast cancer cells.Methods By using Transwell co-culture technique,murine breast cancer 4T1 cells were incubated with RAW264.7 mac-rophages for 5 days in vitro.Cells in the experiment group were pretreated with curcumin.Flow cytometry was used to detect the CD163 and F4/80 expression on macraphages and zymography to analyze the activity of MMP-9 secreted by co-cultured macrophages after treatment in the absence of serum for a night.Migration of macrophages towards breast cancer cell media was investigated by Transwell assay.Additionally,curcumin was orally administrated to mice bearing orthotopic transplantation tumors.Tumor growth was observed as well as overall survival.Furthermore,the CD163+/F4/80+macrophages in orthotopic transplantation tumor was measured by immunofluorescence.Results In vitro experiments showed that curcumin could dra-matically suppress the expression of CD163 and F4/80 on macrophages,the secretion of MMP-9 and the migration of macropha-ges.Furthermore,while the tumor growth was not significantly inhibited by curcumin,in vivo delivery of curcumin could pro-long the overall survival of tumor-bearing mice and downregulate the proportion of CD163+/F4/80+ macrophages in orthotopic transplantation tumors.Conclusion Curcumin could inhibit the macraphage polarization induced by breast cancer cells.