RESUMO
Objective To determine the effect of janus kinase/signal transducer and activator of transcription(JAK/STAT) pathway on the expressions of interferon-?(IFN-?) in vital organs of rats with sepsis.Methods A sepsis model was induced by cecal ligation puncture(CLP) in 60 male Wistar rats,and randomly divided into CLP group(n=24),AG490 group in which inhibitor of JAK-AG490 at dose of 8 mg/kg was subcutaneously injected 30 min before CLP(n=18),and rapamycin group in which inhibitor of STAT-rapamycin at dose of 0.4 mg/kg was subcutaneously injected 30 min before CLP(n=18).On 2,6,24 h after CLP,all rats were sacrificed,then hepatic and pulmonary tissue samples were harvested to determine IFN-? mRNA expression levels by reverse transcription polymerase chain reaction(RT-PCR) and its protein expression levels by enzyme-linked immunosorbent assay(ELISA).Results As compared with normal rats without CLP,IFN-? mRNA and protein levels in hepatic and pulmonary tissues at 2,6,24 h after CLP significantly elevated(P
RESUMO
Objective To evaluate the effect of self-expandable nitinol mesh stent (SENMT) for advanced rectal cancer accompanied with obstruction. Methods Clinical data were retrospectively analysed on 12 patients with advanced rectal cancer accompanied with acute or chronic obstruction treated by the stent placement. Results SENMT was placed successfully in 10 patients including replacement in 2 patients because of the stent was migrated. The bowel movement recovered in all of the 10 patients. Ten patients were followed-up, 5 cases died within 56-720 days, and 5 others survived without intestinal obstruction for 6-15 months. The stent failed to be placed in other 2 patients. Conclusions SENMT may be useful in the management of terminal or high-risk surgical patients for palliative purposes. Palliation management of stent placement combined with chemotherapy and immunotherapy might prolong the survival time of these patients.
RESUMO
Objective To evaluate the effect of self expandable nitinol mesh stent (SENMT) for advanced rectal cancer accompanied with obstruction. Methods Clinical data were retrospectively analysed on 12 patients with advanced rectal cancer accompanied with acute or chronic obstruction treated by the stent placement. Results SENMT was placed successfully in 10 patients including replacement in 2 patients because of the stent was migrated. The bowel movement recovered in all of the 10 patients. Ten patients were followed-up, 5 cases died within 56-720 days, and 5 others survived without intestinal obstruction for 6-15 months. The stent failed to be placed in other 2 patients. Conclusions SENMT may be useful in the management of terminal or high risk surgical patients for palliative purposes. Palliation management of stent placement combined with chemotherapy and immunotherapy might prolong the survival time of these patients.