RESUMO
Objective: Furcal perforation is an undesirable complication that may occur during root canal therapy and seriously compromises the prognosis of endodontic treatment
The prognosis of furcal perforation repair depends on the prevention of bacterial infection of the perforation site. Thus, use of a biocompatible material for complete sealing of the perforation site is critically important. This study aimed to compare the microleakage of mineral trioxide aggregate [MTA] and octacalcium phosphate [OCP] for furcal perforation repair
Methods: This experimental study was conducted on 70 human mandibular molars with divergent roots, intact furcation site and completely formed apices. The teeth were randomly divided into 4 groups of two experimental [n=30], one positive control and one negative control group [n=5]
The furcation floor was then perforated. In the first and second experimental groups, furcation perforations were repaired with OCP and MTA, respectively. In the positive control group, the perforation was left untreated and in the negative control group no furcal perforation was created
The access cavity in all groups was filled with Cavit. The teeth were stored in 100% humidity for 48h. Root apices were sealed with sticky wax and the entire external surface of teeth except for 0.5 mm around the perforation site was covered with two coats of nail polish
The specimens were immersed in India ink for 4 days and then sectioned in half buccolingually
The sections were separated and dye penetration was evaluated using a stereomicroscope at 25X magnification
Obtained data were analyzed using independent t-test
Results: No microleakage was observed in the negative control group. In the positive control group, the dye had completely penetrated into the walls. The amount of dye penetration was 1.64 and 1.22 mm in the OCP and MTA groups, respectively. Statistical analysis of data revealed that the sealability of MTA was significantly greater than that of OCP [p<0.0001]
Conclusion: OCP cannot be an acceptable alternative to MTA in terms of sealability?
RESUMO
Glycoconjugates are a class of glycoproteins or glycolipids, their terminal sugars are responsible for cell-cell and/or cell-extracellular matrix interactions. Aberrant glycosylation of these compounds are one of the most important aspects of cellular transformation, metastasis and escape of tumoral cells from immune system and resistance to antineoplastic drugs. Recent studies showed that patients with HPA [helix pomatia agglutinin] positive intraductal carcinoma cells have worse prognosis compared to patients with HPA negative cells. The aim of the present study was to define the presence of GalNac terminal sugar in glycoconjugate of different grades of intraductal breast carcinoma and to compare the degree and the pattern of reactivity of tumoral cells to HPA lectin. Material and The paraffin blocks belonging to 20 patients of intraductal carcinoma was chosen from pathology archive of Khatam-Al-Anbia hospital in Zahedan and 5-7 micrometer sections were prepared. Two expert pathologists determined histopathological grading independently. The lectin histochemistry was performed using HPA. The same observers determined histochemical grading. Data were analyzed by NPAR [non-parametric] test of Mann Whitney. Results of this study revealed that the pattern and the degree of histochemical reactivity of neoplastic cells differ in all grades of intraductal carcinoma. Histochemical staining showed significant difference between grades of intraductal carcinoma of the breast [p<0.003]. The lowest reactivity was seen in grade I and the highest in grade III. Furthermore, the reaction of tumoral cells was primarily confined to apical surfaces of cells in grade I, to the Golgi zone in grade II, and to a diffuse cytoplasmic distribution in grade III Our data suggest that the HPA reactivity of tumoral cells were different in all grades of intraductal carcinoma. The tumor cells showed aberrant glycosylation, which occurred in the course of anaplastic changes. It seems that our data suggest a potential and clinically important role of HPA reactivity to predict the invasive nature of malignant tumoral cells of intraductal carcinoma of the breast