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Rev. Soc. Bras. Med. Trop ; 48(4): 454-459, July-Aug. 2015. tab, ilus
Artigo em Inglês | LILACS | ID: lil-755974

RESUMO

AbstractINTRODUCTION:

Candida parapsilosis is a common yeast species found in cases of onychomycosis and candidemia associated with infected intravascular devices. In this study, we differentiated Candida parapsilosis sensu stricto, Candida orthopsilosis , and Candida metapsilosis from a culture collection containing blood and subungual scraping samples. Furthermore, we assessed the in vitro antifungal susceptibility of these species to fluconazole, itraconazole, voriconazole, posaconazole, amphotericin B, and caspofungin.

METHODS:

Differentiation of C. parapsilosis complex species was performed by amplification of the secondary alcohol dehydrogenase (SADH) gene and digestion by the restriction enzyme Ban I. All isolates were evaluated for the determination of minimal inhibitory concentrations using Etest, a method for antifungal susceptibility testing.

RESULTS:

Among the 87 isolates, 78 (89.7%) were identified as C. parapsilosis sensu stricto , five (5.7%) were identified as C. orthopsilosis , and four (4.6%) were identified as C. metapsilosis . Analysis of antifungal susceptibility showed that C. parapsilosis sensu strictoisolates were less susceptible to amphotericin B and itraconazole. One C. parapsilosis sensu stricto isolate was resistant to amphotericin B and itraconazole. Moreover, 10.2% of C. parapsilosis sensu stricto isolates were resistant to caspofungin. Two C. parapsilosis sensu strictoisolates and one C. metapsilosis isolate were susceptible to fluconazole in a dose-dependent manner.

CONCLUSIONS:

We reported the first molecular identification of C. p...


Assuntos
Adulto , Humanos , Antifúngicos/farmacologia , Candida/efeitos dos fármacos , Candida/classificação , Farmacorresistência Fúngica , Testes de Sensibilidade Microbiana , Técnicas de Tipagem Micológica/métodos
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