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Rev. Inst. Med. Trop. Säo Paulo ; 41(4): 239-42, July-Aug. 1999.
Artigo em Inglês | LILACS | ID: lil-246833

RESUMO

The authors studied 70 leprosy patients and 20 normal individuals, comparing the traditional sera collection method and the finger prick blood with the conservation on filter paper for specific antibodies against the native phenolic glycolipid-I (PGL-I) from Mycobacterium leprae. The finger prick blood dried on filter paper was eluated in phosphate buffer saline (PBS) containing 0.5 percent gelatin. The classical method for native PGL-I was performed for these eluates, and compared with the antibody determination for sera. It was observed that there is a straight correlation comparing these two methods; although the titles found for the eluates were lower than those obtained for serology. This blood collection method could be useful for investigation of new leprosy cases in field, specially in contacts individuals


Assuntos
Humanos , Feminino , Adolescente , Idoso , Pessoa de Meia-Idade , Adulto , Anticorpos Antibacterianos/imunologia , Antígenos de Bactérias/imunologia , Coleta de Amostras Sanguíneas/métodos , Glicolipídeos/sangue , Hanseníase/imunologia , Mycobacterium leprae/imunologia , Anticorpos Antibacterianos/sangue , Antígenos de Bactérias/sangue , Ensaio de Imunoadsorção Enzimática , Hanseníase/sangue
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