RESUMO
INTRODUCTION: Conventional homeopathy advocates giving 10 strokes (agitation/succussions) to a prescription bottle containing liquid dilution, before taking it. To scientifically support such activity, it must be based on studies on the physicochemical aspects of the memory of water and agitated water chains, supplemented with spectrometry. OBJECTIVE: To analyze spectrometric profiles of homeopathic high dilutions, comparing additionally agitated and non-agitated dilutions, and to define differences according to each dilution´s kingdom. METHODOLOGY: Homeopathic dilutions were prepared using a ratio of 1:100 obtaining dilutions from 1cH to 15cH. Measurements of absorbance of 15cH dilutions were performed at 340 nm and 200 nm, with and without additional agitation. RESULTS: Statistically significant differences were found between agitated and non-agitated dilutions (p <0.05) for Apis mellifica, Bufo rana, and Calcium iodatum, with a decrease in the mean value of absorbance (340 nm) in agitated solutions. For A nica montana, Matricaria chamomilla, Coffea arabica, Pulsatilla nigricans, Calcium iodatum and Natrum muriaticum, the absorbance values increased; these values decreased for Apis and Bufo. In addition, measurements were performed with a shorter wavelength (200 nm); it was observed that there are significant differences for all homeopathic dilutions studied. Additionally, when comparing the mean values of the absorbance for each kingdom, and observing the agitation factor, a statistically significant difference was found in the animal kingdom (p<0.05), having lower absorbance for agitated dilutions. CONCLUSION: Comparisons of the spectrometric profiles of agitated and non-agitated solutions allow to observe that there is a difference of the absorbance when comparing a previously agitated solution and a solution with no agitation before taking it.