RESUMO
OBJECTIVE: This study aims to evaluate the production of interferon-gamma and interleukin-10 by stimulated peripheral blood mononuclear cells isolated from patients with supraglottic laryngeal cancer before and after surgical treatment. METHODS: Fourteen patients with advanced supraglottic laryngeal cancer were studied. Cultures of peripheral blood mononuclear cells isolated during the preoperative and late postoperative periods were stimulated with concanavalin A and Bacille Calmette-Guerin, and the supernatant concentrations of interferon-gamma and interleukin-10 were measured. RESULTS: For non-stimulated cultures, the interferon-gamma levels produced by the preoperative period and the late postoperative period cultures were lower than the levels produced by the control group cultures. The interferon-gamma levels after stimulation with concanavalin A were higher in the late postoperative period cultures than in the preoperative evaluation cultures. Stimulation with Bacille Calmette-Guerin led to the production of similar levels of interferon-gamma and interleukin-10 by all cultures; thus, stimulation increased the levels of interferon-gamma produced by both the preoperative and postoperative cultures relative to the levels produced by the corresponding unstimulated cultures. CONCLUSION: Patients with advanced supraglottic laryngeal cancer exhibit an in vitro deficiency in interferongamma secretion by mononuclear cells. Stimulated cells seem to recover this function during the postoperative period.
Assuntos
Adolescente , Adulto , Criança , Feminino , Humanos , Masculino , Adulto Jovem , Carcinoma/sangue , Interferon gama/biossíntese , /biossíntese , Neoplasias Laríngeas/sangue , Leucócitos Mononucleares/metabolismo , Estudos de Casos e Controles , Carcinoma/patologia , Concanavalina A/farmacologia , Citocinas/sangue , Interferon gama/sangue , /sangue , Neoplasias Laríngeas/patologia , Leucócitos Mononucleares/efeitos dos fármacos , Mycobacterium bovis , Mitógenos/farmacologia , Estadiamento de Neoplasias , Estatísticas não ParamétricasRESUMO
A cetoacidose diabética é uma complicação aguda, típica dos pacientes com DM tipo I ou insulinodependentes, um conjunto de distúrbios metabólicos, que se desenvolvem em uma situação de deficiência insulínica grave, comumente associada a condições estressantes, que levam a aumento dos hormônios contra-reguladores. O estado hiperglicêmico hiperosmolar é uma complicação aguda, característica do diabético tipo II ou não insulinodependente, quando predominam os efeitos da hiperosmolaridade e desidratação, principalmente envolvendo o sistema nervoso central. Fatores precipitantes, quadro clínico, fisiopatologia básica e tratamento dessas emergências médicas são analisados nesta revisão