Função motora e qualidade de vida de indivíduos com paralisia cerebral / Motor function and life quality of individuals with cerebral palsy

OBJETIVO: Avaliar e correlacionar a função motora grossa e a qualidade de vida de crianças com PC. MÉTODOS: Foi verificado em 20 crianças o tipo clínico e anatômico, a função motora grossa pela GMFM (Gross Motor Function Measure) e a qualidade de vida pelo Questionário de Qualidade de Vida Pediátrico (PedsQL). Foi utilizado o teste de Shapiro-Wilk e o de Kruskal-Wallis. Para correlacionar utilizou-se da Análise de Correlação Canônica, o teste da Razão de Verossimilhança e a Estatística Multivariada de Wilks Lambda, com nível de significância de 5%. RESULTADOS: Apresentaram idade média de 8,4±4,03 anos. Os indivíduos com hemiparesia espástica apresentaram função motora significativamente maior que os com tetraparesia atetóide e espástica. Verificou-se a existência de alta correlação canônica significativa entre as variáveis da GMFM e PedsQL. CONCLUSÃO: Os achados demonstram que quanto maior for o comprometimento da função motora grossa menor será a sua qualidade de vida.

OBJECTIVE: The objective of this study was to evaluate and correlate the gross motor function and the quality of life of children with cerebral palsy. METHODS: Clinical and anatomical type, the gross motor function through the Gross Motor Function Measure and the quality of life were verified in 20 subjects through questionnaire for quality of Pediatric Life. The Shapiro-Wilk's test and the Kruskal-Wallis were used for the analyses. To correlate, we used the Canonical Correlation Analysis, the Likelihood Ratio test and Wilk's Lambda Multivariate Statistics, with significance level of 5%. RESULTS: The average age of the subjects was 8.4±4.03 years. Individuals with spastichemiparesis showed significantly greater motor function than those classified as athetoid quadriplegia and spastic. The existence of highly significant canonical correlation was found between the variables relating to the Gross Motor Function Measure and quality of Pediatric Life. CONCLUSION: The findings show that the greater the impairment of gross motor function the lower the qualify of life.

Cardiac markers: profile in rats experimentally infected with Toxocara canis / Marcadores cardíacos: perfil em ratos infectados experimentalmente com Toxocara canis

The aim of this study was to evaluate the profile of the enzymes creatine kinase (CK), creatine kinase MB (CK-MB) and lactate dehydrogenase (LDH) in Wistar rats infected with 250 (GI, n = 24) or 1000 (GII, n = 24) Toxocara canis eggs. Animals were evaluated on days 7, 15, 30, 60, 120 and 180 post-infection (DPI). Only the GI rats showed an increase in CK and CK-MB, at 15 and 30 DPI, respectively. Anti-Toxocara spp. antibodies were detected by ELISA in infected animals. Despite of the presence of eosinophilic infiltrate in the heart of three infected animals, none larva was recovered from the organ neither by acid digestion nor by Baermann procedure. Eosinophilia was observed in both groups but there was no significant difference in the eosinophil counts between GI and GII (p = 0.2239). It is possible to consider that cardiac lesion is an eventual finding in murine model for toxocariasis

O objetivo do presente estudo foi avaliar o perfil das enzimas creatinoquinase (CK), creatinoquinase-MB (CK-MB) e lactato desidrogenase (LDH) em ratos Wistar infectados com 250 (GI, n = 24) ou 1000 (GII, n = 24) ovos de Toxocara canis. Os animais foram avaliados nos dias 7, 15, 30, 60, 120 e 180 pós-infecção (DPI). Observou-se que apenas os animais do GI apresentaram aumento da atividade de CK e CK-MB aos 15 e 30 DPI, respectivamente. Anticorpos anti-T. canis foram detectados por ELISA nos animais infectados. Apesar da presença de infiltrado eosinofílico em três animais infectados, nenhuma larva foi recuperada do coração pela digestão ácida ou pela técnica de Baermann. Eosinofilia foi observada em todos os momentos em GI e GII, sem diferença significativa entre os grupos (p = 0,2239). Pode-se considerar que as lesões cardíacas foram um achado eventual no modelo murino para toxocaríase.

Skeletal muscles with antagonistic muscular actions: morphological, contractile and metabolic characteristics / Músculo esquelético con acción muscular antogonista: características metabólicas, contratiles y morfológicas

The muscles can perform the same function in a specific segment (muscles of fast and slow contraction), and at the same time be antagonistic in relation to muscular action (flexors or extensors). The present research aimed to study the morphology, frequency and metabolism of fiber types and the contractile characteristics of extensor and flexors muscles of rabbit. We studied muscles anterior tibialis (AT), flexor digitorum supeficialis (FDS), extensor digitorum longus (EDL) and posterior tibialis (PT). The muscles were submitted to the techniques HE, NADH-TR and myofibrillar ATPase. In EDL and PT extensor muscles, the frequencies of red (SO + FOG) and white fibers (FG) were 68.77 percent and 31.23 percent versus 58.87 percent and 41.13 percent, respectively. In the AT and FDS flexor muscles, these frequencies were 75.14 percent and 24.86 percent versus 73.89 percent and 26.11 percent, respectively. In extensor muscles, the percentage of slow contraction fibers was 8.05 percent in EDL and 9.74 percent in PT, and in fast contraction, 91.95 percent in EDL and 90.26 percent in PT. In flexors, the slow contraction frequencies were 12.35 percent in AT and 8.17 percent in FDS, and in fast contraction, 87.65 percent and 91.83 percent, respectively. Skeletal muscles with antagonistic muscular actions (flexors and extensors) the morphological, contractile and metabolic characteristics are identical.

Los músculos estriados esqueléticos pueden desempeñar la misma función en un segmento corporal específico (músculos decontracción rápida y lenta), y al mismo tiempo pueden ser antagonistas en relación a su acción muscular (flexores o extensores). El objetivo de esta investigación fue analizar la morfología, el metabolismo y la frecuencia de los tipos de fibras y las características contráctiles de músculos flexores y extensores del conejo. Fueron evaluados los músculos tibial anterior (TA), flexor superficial de los dedos (FDS), extensor largo de los dedos (EDL) y tibial posterior (TP). Los músculos fueron sometidos a las técnicas de Hematoxilina Eosina (HE), NADH-TR y ATPase miofibrilar. En los músculos extensores EDL y TP, las frecuencias de los tipos de fibras rojas (SO + FOG) y fibras blancas (FG) fueron de 68,77 por ciento y 31,23 por ciento versus 58,87 por ciento y 41,13 por ciento, respectivamente. En los músculos flexores TA y FDS estas frecuencias fueron 75,14 por ciento y 24,86 por ciento versus 73,89 por ciento y 26,11 por ciento, respectivamente. En los músculos extensores el porcentaje de fibras de contracción lenta fue 8,05 por ciento en el EDL y 9,74 por ciento en el TP; y las fibras de contracción rápida fue de 91,95 por ciento en el EDL y 90,26 por ciento en el TP. En los flexores la frecuencia de fibras de contracción lenta fue12,35 por ciento en el TA y 8,17 por ciento FSD, y las fibras de contracción rßpida fue 87,65 por ciento y 91,83 por ciento respectivamente. Músculos esqueléticos con acción muscular antagónica (flexores y extensores) poseen características morfológicas, contráctiles y metabólicas idénticas.